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Bordetella - Respiratory
SPECIMEN REQUIREMENTS
Specimen Type:

​Nasopharyngeal Swab (NPS)

Container/Kit:

Bordetella pertussis BP collection kit

Collection information:

​To order Collection Kits or other PHOL Supplies complete the Requisition for Containers and Supplies. The form should be faxed to Public Health Ontario Laboratory, Toronto at 416-235-5753 or your local PHOL.

Minimum volume required:

​None

Requisition:

​Complete all fields of the General Test Requisition Form. Ensure the following fields are completed if diagnostic testing is requested:

• Symptoms
• Onset date

Limitation:

​The PCR transport medium is colourless. NB: Do not use Multi-organism transport medium which is pink.

SPECIMEN HANDLING
Preparation prior to transport:

​Label the container with two identifiers and place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs. Maximum storage/transit time at 2-8 degrees C is within 5 days of collection.

Special Instructions:

​Follow the instructions found in the Bordetella pertussis BP Kit Instruction Sheet​.  Specimens should be collected during the acute phase of illness.

TEST INFORMATION

​Bordetella - respiratory specimens are tested by a (PCR)- Polymerase Chain Reaction test that detects Bordetella Pertussis, Bordetella parapertussis and Bordetella holmesi. The PCR is >99% specific, and more sensitive than routine culture.

ADDITIONAL INFORMATION
TESTING FREQUENCY AND TURNAROUND TIME (TAT)

​The PCR is set up daily. 

TAT for PCR testing is up to 4 days.

REPORTING

​Results are reported to the ordering physician or health care provider as indicated on the requisition.


Uncontrolled print copy. Valid only on day of Print: [date]
Page updated on [date/time] 03/06/2016 3:15 PM
© , Ontario Agency for Health Protection and Promotion