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Enteric Bacteria – Speciation
SPECIMEN REQUIREMENTS
Specimen Type:

​Pure viable subculture of organism on media that supports the growth.

Container/Kit:

​Pure bacteria in appropriate agar plate, tube or swab for identification, confirmation and serotyping.

Collection information:

​None

Minimum volume required:

​None

Requisition:

​Complete all fields of the Reference Bacterial Requisition form.

Ensure the following fields are completed:

  • ​Test/s Requested
  • Culture Information
  • Clinical/epidemiological information

Limitation:

​None

SPECIMEN HANDLING
Preparation prior to transport:

​Place specimen in a biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Special Instructions:

​None

TEST INFORMATION

​Specimens are tested by conventional bacterial culture. Bacterial identification includes biotyping and serotyping procedures.

Antimicrobial susceptibility testing is routinely performed on Salmonella Typhi, Salmonella Paratyphi, Shigella, Vibrio and for clinical indications (from blood or sterile sites, infants <1 year old, immuno-compromised patients and hospitalized patients–if documented).

Antimicrobial susceptibility testing on other bacteria is available upon request.

ADDITIONAL INFORMATION

Bacterial identification, confirmation and serotyping tests are applicable for Salmonella, Shigella, Campylobacter, pathogenic Yersinia, E.coli O157:H7 and non-O157 shiga toxin producing E. coli (STEC), Vibrio and other gram negative fermenters.


For outbreaks, contact your local Health Unit, your local Public Health Laboratory or the Public Health Ontario, Public Health Laboratory Customer Service at 416-235-6555 or 1-877-604-4567.

TESTING FREQUENCY AND TURNAROUND TIME (TAT)

​Enteric bacterial speciation is performed Monday to Friday.

TAT is up to 10 days.

REPORTING

​Results are reported to the ordering physician or health care provider as indicated on the requisition.

Specimens that are positive for Salmonella, Shigella, Campylobacter, pathogenic Yersinia, E.coli O157:H7 and other shiga toxin producing E. coli, and Vibrio are reported to the Medical Officer of Health as per the Health Protection and Promotion Act.


Uncontrolled print copy. Valid only on day of Print: [date]
Page updated on [date/time] 26/01/2017 1:20 PM
© , Ontario Agency for Health Protection and Promotion