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Group A Streptococci (GAS) - Pulse Field Gel Electrophoresis (PFGE)
SPECIMEN REQUIREMENTS
Specimen Type:

​N/A

Container/Kit:

​Pure bacterial culture of pathogen in appropriate agar

Collection information:

​To order Collection Kits or other PHOL Supplies complete the Requisition for Containers and Supplies. ​The form should be faxed to Public Health Ontario Laboratory, Toronto at 416-235-5753 or your local PHOL.

Minimum volume required:

​N/A

Requisition:

​Complete the Reference Bacteriology Requisition. Complete all fields on the requisition.  Indicate on the requisition the outbreak number and index case where applicable

Limitation:

​None

SPECIMEN HANDLING
Preparation prior to transport:

​Label the specimen with 2 patient identifiers and place in individual biohazard bag and seal

Special Instructions:

​None

TEST INFORMATION

​All GAS submissions are confirmed as such prior to PFGE testing

ADDITIONAL INFORMATION

​GAS PFGE is only performed in outbreak situations. Indication of the index case is highly recommended.  Molecular typing for special circumstances must be approved by a PHOL Microbiologist at 416-235-6556 or 1-877-604-4567

TESTING FREQUENCY AND TURNAROUND TIME (TAT)

​The GAS PFGE test is performed daily Monday to Friday

TAT is up to 10 days.

REPORTING

​Results are reported to the ordering physician or health care provider as indicated on the requisition


Uncontrolled print copy. Valid only on day of Print: [date]
Page updated on [date/time] 08/05/2013 2:33 PM
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