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Hepatitis A – Genotyping-Subtyping
SPECIMEN REQUIREMENTS
Specimen Type:
​Serum or plasma
Container/Kit:
​Blood, clotted - vacutainer tubes (SST) or EDTA tubes
Collection information:
​To order Collection Kits or other PHL Supplies complete the Requisition for Containers and Supplies. Toronto area clients should fax the completed order form to 416-235-5753.
Minimum volume required:
​5.0 ml blood or 1.0 ml serum/plasma
Requisition:

​Complete all fields of the General Test Requisition Form.

External Labs must indicate on the requisition that specimen has already been tested for HAV IgM and that the result is HAV IgM Positive.

Limitation:
​Haemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing
SPECIMEN HANDLING
Preparation prior to transport:

​Centrifuge the SST/EDTA tube and transfer serum/plasma to a cryovial. Store serum/plasma specimen frozen at <-600C following collection.  Place specimen in biohazard bag and seal. Ship to PHL frozen on dry ice or ice packs. Minimum specimen volume to be forwarded to NML is 1.0 mL.

Special Instructions:
​Instructions for using SST tubes are found in the document titled: Blood Collection Using Serum Separator Tubes.
TEST INFORMATION

​Hepatitis A - Genotyping Tests are referred to the Public Health Agency of Canada-National Microbiology Lab (NML).

Hepatitis A Genotype testing is performed by conventional RT- Polymerase Chain Reaction (PCR) followed by sequencing.

ADDITIONAL INFORMATION

NML contact: Molecular and Immunodiagnostics Section, Bloodborne Pathogens and Hepatitis
                            Phone: (204) 789-6062
                            Fax: (204) 789-2082
                            Email: NMLBloodbornePathogens@phac-aspc.gc.ca  

TESTING FREQUENCY AND TURNAROUND TIME (TAT)

​Hepatitis A - genotyping specimens are shipped to NML weekly. 

TAT maybe up to 21 days.

REPORTING
​Results are reported to the ordering physician or health care provider as indicated on the requisition. Results are also reported to local MOH where the patient is residing.

Uncontrolled print copy. Valid only on day of Print: [date]
Page updated on [date/time] 31/03/2014 9:14 AM
© , Ontario Agency for Health Protection and Promotion