Nasopharyngeal swab, throat swab, bronchoalveolar lavage (BAL), pleural fluid, respiratory tract tissue
Virus-Respiratory Collection Kit. Nasopharyngeal aspirates, pleural fluid, respiratory tract tissue or BALs may be submitted in sterile containers.
The recommended respiratory specimen is a nasopharyngeal swab or aspirate.
To order Collection Kits or other PHOL Supplies complete the Requisition for Containers and Supplies. The form should be faxed to the Public Health Ontario Laboratory at 416-235-5753 or your local PHOL.
Submit 1 swab or 2.0 ml aspirate
Complete all fields of the General Test Requisition Form. Ensure the following fields are completed if diagnostic testing is requested:
• Patient setting (ICU, hospitalized, ER not admitted)• Symptoms • Onset date• Travel history • Animal exposure; contact PHOL Customer Service if submitting specimens from a patient with respiratory symptoms of onset within 10 days of swine or avian exposure
Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.
Follow the instructions found in the Virus-Respiratory Kit Instruction Sheet.
Refer to the current labstract on respiratory viral testing titled: LAB-SD-076 (Spring/Summer) and/or LAB-SD-070 (Fall/Winter) for testing method that will be performed based on patient setting and season. Testing methods include rapid antigen detection, rapid respiratory virus culture (shell vial) or traditional virus culture, influenza A/B PCR, and multiplex respiratory viral PCR (MRVP).
For Outbreaks, contact your local Health Unit.
The Influenza testing is performed daily Monday to Friday.PCR testing on the weekend will be done under special circumstances.The TAT for the Direct Antigen Test for Influenza is within 1 day after receipt.The TAT for Influenza A/B PCR is up to 2 days after receipt at testing site.The TAT for MRVP is up to 3 days after receipt at testing site.The TAT for rapid respiratory virus culture (shell vial) is up to 4 days.Samples tested by virus culture are observed for a minimum of 10 days prior to reporting a negative culture result; samples that are positive can be observed at any point during this period; TAT is up to 12 days.TAT will vary during the winter months.
Results are reported to the ordering physician or health care provider as indicated on the requisition.Specimens that are positive for Influenza are reported to the Medical Officer of Health as per Health Protection and Promotion Act.