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Mumps - Immunity Serology
SPECIMEN REQUIREMENTS
Specimen Type:

​​Serum  or Plasma

Container/Kit:

​Blood, clotted - vacutainer tubes (SST) 

Collection information:

​To order Collection Kits or other PHOL Supplies complete the Requisition for Containers and Supplies. The form should be faxed to Public Health Ontario Laboratory, Toronto at 416-235-5753 or your local PHOL.

Minimum volume required:

​5.0 ml blood or 1.0 ml serum or plasma

Requisition:

​Complete all fields of the General Test Requisition Form

NB: Only check the box marked “Immune Status” when immune status testing is required. Do not check “Diagnostic” or both boxes. If “Diagnostic” is ticked   both IgG and IgM testing will be performed.  IgM is not needed for the determination of immune status as it is a marker of acute infection.

Limitation:

​Hemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.

SPECIMEN HANDLING
Preparation prior to transport:

​Centrifuge if using SST. Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Special Instructions:

​Instructions for using SST tubes are found in the document titled: LAB-SD-008, Blood Collection Using Serum Separator Tubes.

TEST INFORMATION

​The Mumps IgG test is performed using an ELISA (Enzyme Linked Immunosorbent Assay) test kit manufactured by Euroimmun Diagnostics (Germany). This ELISA test provides a quantitative in vitro assay for human antibodies of the IgG class against mumps  virus in serum or plasma.
Mumps IgG test results are reported both qualitatively (Non-Reactive, Indeterminate or Reactive) and quantitatively together with an interpretation of the patient’s immune status based on the level of mumps IgG detected.
The unit of mumps IgG antibody measurement is RU/ml (Relative units/millilitre).

Mumps IgG Antibody Ranges and Result Interpretation:

Qualitative Result​​​​ Quantitative  Result​ Interpretation​
​Non-reactive ​<16 RU/ml ​No Evidence of Immunity.(Indicates susceptibility
Indeterminate​ ​≥16 - <22 RU/ml ​Antibody levels in the indeterminate range for this assay may be insufficient to confer immunity.
Reactive​ ​≥22 RU/ml ​Evidence of past infection / vaccination; evidence of immunity.
 
 
N.B. These interpretations assume the absence of acute or recent infection

Quantitative results will ONLY be provided when the reason for testing is for the determination of immunity / immune status and this is clearly indicated on the PHOL requisition. If diagnosis is indicated or ticked quantitative IgG results will not be reported.
ADDITIONAL INFORMATION
TESTING FREQUENCY AND TURNAROUND TIME (TAT)

​Mumps IgG serology is performed daily Monday to Saturday.

The TAT for test results is up to 5 days for routine testing. 

REPORTING

​Results are reported to the ordering physician or health care provider as indicated on the requisition.


Uncontrolled print copy. Valid only on day of Print: [date]
Page updated on [date/time] 16/06/2016 9:17 AM
© , Ontario Agency for Health Protection and Promotion