COVID-19 PCR Collection Kits

The following chart provides information on collection kits for procurement that are acceptable for COVID-19 PCR testing.

COVID-19 Polymerase Chain Reaction (PCR) Testing Swabs: Preferred Specimen Collection Methods

PHO Laboratory has done a limited evaluation on the specimen collection kits listed. These can be used for collection of intended or alternative specimens as indicated in the table. The same specimen can be used for seasonal respiratory virus testing (MRVP) provided acceptance criteria are met.

PHO Laboratory will accept other swab types and other liquid transport media (except gel or solid media) for COVID-19 testing; however, if the submitted swab and/or media type were not validated at PHO Laboratory for COVID-19 testing, the results will be reported with a disclaimer. Further information on collection kits is available in the COVID-19 Laboratory Testing FAQs for SARS-CoV-2.

At this time there are no dedicated neonatal nasopharyngeal swabs available in Ontario. Current advice is to collect an NP swab when testing neonates for COVID-19. If the calibre of the swab that is available is too wide, it can be used for nasal, deep nasal or throat swab collection as alternative collection sites.

If you are ordering collection kits from Ontario Health Digital Health Services page, any of these kits may be used to fulfill orders.

Specimen Collection Kits and Instructions

Swab-Media Kit (Catalogue#) Description Specimens (Intended and Alternative) Suitable for
(R=Flu Rapid**; C=COVID-19; M=Multiplex)

Saliva - neat – funnel

Neat with funnel kit

A sterile empty tube and funnel

Media Type: No Media

Saliva - NEAT C

Saliva – neat - straw

Neat with straw kit

A sterile empty tube and straw

Media Type: No Media

Saliva - NEAT C

Saliva: mouth rinse – “swish and gargle” – funnel

Swish and Gargle with Funnel Kit

A sterile empty tube, funnel, and 5 ml packet of saline rinse

Media Type: no media

Saliva - mouth rinse/“swish and gargle” C

Saliva: mouth rinse – “swish and gargle” – straw

Swish and Gargle with straw kit

A sterile empty tube, straw, and 5 ml packet of saline rinse

Media Type: no media

Saliva - mouth rinse/“swish and gargle” C

BD Kit (B220528)

BD - B220528

3mL vial of media and sterile nylon flocked tip swab (100mm breakpoint)

Media Type: VTM/UTM

Intended:

C,M

BD Kit (B220531)

 

3mL vial of media and sterile mini-tip swab

Media Type: VTM/UTM

Intended:

Alternative:

C,M

Beaver Kit (43903)

Beaver Biomedical Engineering - 43903

3mL vial of clear media and flocked “specimen collection swab” (80mm breakpoint)

Media Type: Buffered saline***

Intended:

Alternative:

R,C,M

Copan Kit (CA302MH)

Copan - CA302MH

3mL vial of media and standard flocked swab (100mm breakpoint)

Media Type: VTM/UTM

Intended:

R,C,M

Copan Kit (CA305MH)

Copan - CA305MH

3mL vial of media and pernasal flocked swab (100mm breakpoint)

Media Type: VTM/UTM

Intended:

Alternative:

R,C,M

Copan Pediatric Midturbinate Swab Kit (CA56750CS01)

Copan - CA56750CS01

3mL vial of media and pediatric midturbinate flocked swab (55mm breakpoint)

Media Type: VTM/UTM

Intended:

Alternative:

R,C,M

Deaou Kit

Deaou Kit - Reworked

3mL vial of media and “disposable sampling swab” (80mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

ESBE EZ Pro Kit (ESB-VA50005NP)

ESBE  EZPro ESBVA50005NP

EZ Pro Transport Medium Swab Kit w/ 2mL vial of clear media and “sterile collection swab” (85mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

GDL Korea Kit

GDL Korea

2mL vial of pink media and nylon flocked swab (95mm breakpoint)

Media Type: VTM/UTM***

Intended:

Alternative:

R,C,M

Hologic (Aptima) Multitest Swab Kit (PRD-03546)

Hologic Multitest Swab

Multitest Swab Specimen Collection Kit w/ 2.9 mL vial of media and woven multitest swab (75mm breakpoint)

Media Type:Specimen Transport Media***

Intended:

C,M

Hologic (Aptima) Unisex Swab Kit (301041)

Hologic Uni Swab

Unisex Swab Specimen Collection Kit w/ 2.9mL vial of media and woven collection swab (75mm breakpoint) Note: Kit also contains a cleaning swab that is not suitable for testing.

Media Type:Specimen Transport Media***

Intended:

C,M

McMaster Molecular Medium paired with Miraclean swab

Miraclean with MMM

2mL vial of McMaster Molecular Medium Miraclean flocked “disposable sampling swab” (95mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Miraclean Kit

Miraclean MANTACC

Disposable Sampling Kit w/ 2mL vial of pink media and flocked  “disposable sampling swab” (95mm breakpoint)

Media Type: PBS***

Intended:

Alternative:

R,C,M

Norgen Media paired with i-Clean swab (68803 + CY-96000)

Norgen Media  iClean CY96000

3mL vial of media and narrow-tipped flocked swab (90mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternattive:
C,M

Norgen media paired with i-Clean swab (68803 + CY-93050)

Norgen Media  iClean CY93050

3mL vial of media and flocked “specimen collection swab” (78mm breakpoint).

Media Type: Guanidine/ Guanidinium-based***

Intended:

C,M
Oxoid Kit (OXRE12568)

M6 Kit w/ 3mL vial of media and traditional-tipped flocked swab

Media Type: VTM/UTM

Intended:

R,C,M

Oxoid Kit (OXRE12569)

Oxoid - OXRE12569

M6 Kit w/ 3mL vial of media and micro-tipped flocked swab

Media Type: VTM/UTM

Intended:

Alternative:

R,C,M

Oxoid media paired with Puritan NP swab (R12505 + 25-3317-H)

Oxoid  Remel M4RT  Puritan 253317H

3mL of Remel M4RT media Puritan sterile flocked swab (105mm breakpoint) Media Type: VTM/UTM

Intended:

Alternative:

R,C,M

PHO in-house media paired with Copan swab (220252)

PBS Media paired with Copan Floq

3mL of PHO media Note: Media cap may vary in colour and vial may vary in shape Copan FLOQ swab (100mm breakpoint)

Media Type: PBS***

Intended:

Alternative:

R,C,M

PHO in-house media paired with i-Clean swab (CY-96000)

PBS Media paired with Norgen iClean CY96000

3mL of PHO media Note: Media cap may vary in colour and vial may vary in shape i-Clean narrow-tipped flocked swab (80mm breakpoint)

Media Type: PBS***

Intended:

Alternative:

R,C,M

PHO in-house media paired with Puritan swab (25-3317-H)

PBS Media paired with Puritan253317H

3mL of PHO media Note: Media cap may vary in colour and vial may vary in shape Puritan sterile flocked swab (105mm breakpoint)

Media Type: PBS***

Intended:

Alternative:

R,C,M

Roche Dual Swab Kit (07958021190)

Roche Dual Swab

Cobas PCR Media Dual Swab Sample Packet w/ 4.3mL vial of media, 1 woven tip swab, and 1 flocked tip swab (70mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Roche Uni Swab Kit (07958030190)

Roche Uni Swab

Cobas PCR Media Uni Swab Sample Packet w/ 4.3mL vial of media and woven tip swab (70mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

C,M

Roche + CHS Woven Swab Kit (85-1051)

Roche  CHS  851051

4.3mL of Roche Cobas PCR Media w/ woven tip swab (70mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

C,M

Roche + CHS Flocked Swab Kit (85-1052)

Roche  CHS  851052

4.3mL of Roche Cobas PCR Media w/ flocked tip swab (70mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Roche media paired with Copan swab (06466281190 + 220252)

Roche Cobas PCR media paired with Copan Floq

4.3mL of Roche Cobas PCR Media Copan FLOQ swab (100mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Specimens (Intended and Alternative)

Intended:

Alternative:

C,M

Roche media paired with i-Clean swab (06466281190 + CY-96000)

Roche Cobas PCR media paired with iClean

4.3mL of Roche Cobas PCR Media i-Clean narrow-tipped flocked swab (80mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Roche media paired with Miraclean swab (06466281190)

Roche Cobas PCR media paired with Miraclean

4.3mL of Roche Cobas PCR Media Miraclean flocked “disposable sampling swab” (95mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Roche media paired with Puritan swab (06466281190 + 25-3317-H)

Roche Cobas PCR media paired with Puritan253317H

4.3mL of Roche Cobas PCR Media Puritan sterile flocked swab (105mm breakpoint)  

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Roche media paired with Sensor Health swab (06466281190)

Roche Cobas PCR media paired with Sensor Health swab

4.3mL of Roche Cobas PCR Media Sensor Health flocked “sampling swab” (80mm breakpoint)

Media Type: Guanidine/ Guanidinium-based***

Intended:

Alternative:

C,M

Sensor Health Kit

Sensor Health kit

3mL vial of media and unscored foam-tipped swab w/ sterile scissors

Media Type: PBS***

Intended:

Note: Swab does not have a breakpoint; must be cut manually Steps for cutting swab

R,C,M

Starplex Multitrans Kit (CA66410-030)

Starplex VWR

Starswab Multitrans System w/ 2mL vial of pink media and woven sterile collection swab (100mm breakpoint)

Media Type: VTM/UTM***

Intended:

R,C,M

Trinity Biotech media paired with Beaver swab (B1029-90D)

Trinity Bio paired with Beaver

2mL of Bartels FlexTrans media Note: Cap may vary in colour Beaver flocked “specimen collection swab” (80mm breakpoint)

Media Type: VTM/UTM***

Intended:

Alternative:

R,C,M

Trinity Biotech media paired with i-Clean swab (B1029-90D + CY-96000)

Trinity Bio paired with i-Clean

2mL of Bartels FlexTrans media Note: Cap may vary in colour i-Clean narrow-tipped flocked swab (80mm breakpoint)

Media Type: VTM/UTM***

Intended:

Alternative:

R,C,M

Trinity Biotech media paired with Miraclean swab (B1029-90D)

Trinity Bio paired with Miraclean

2mL of Bartels FlexTrans media Note: Cap may vary in colour Miraclean flocked “disposable sampling swab” (95mm breakpoint)

Media Type: VTM/UTM***

Intended:

Alternative:

R,C,M

Trinity Biotech media paired with Sensor Health swab (B1029-90D)

Roche Cobas PCR media paired with Sensor Health swab

2mL of Bartels FlexTrans media Note: Cap may vary in colour Sensor Health flocked “sampling swab” (80mm breakpoint)

Media Type: VTM/UTM***

Intended:

Alternative:

R,C,M

Yocon Kit (STC-110)

Yocon- Virus sampling kit with VTM

3.4mL vial of pink media and Yocon “ flocked swab” (80mm breakpoint)

Media Type: VTM/UTM***

Intended:

Alternative:

R,C,M

*For instructions on upper respiratory specimen collection, please follow the US CDC’s information for health professionals influenza specimen collection poster.

**Flu Rapid refers to the immunochromatographic rapid influenza test used for institutional outbreak testing; for more information refer to the Respiratory Viruses Test Information Sheet (including influenza) for Flu outbreak testing algorithm.

***Safety Data Sheets available upon request.

Updated 12 Nov 2020