Mumps – Immunity Serology
Serology for Mumps Immunity Serology (Mumps IgG) may be indicated to determine immune status (either following natural infection or post-vaccination).
Diagnostic requests for symptomatic patients or post exposure, refer to the test information sheets Mumps-Diagnostic PCR, and Mumps-Diagnostic Serology for specimen collection and testing information.
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
Whole blood or serum
5.0 ml whole blood or 1.0 ml serum
Blood, clotted - vacutainer tubes (SST)
Lavender top tube
Submission and Collection Notes
Test performed will be Mumps IgG
Check the box marked “Immune Status” on the General Test Requisition Form when immune status testing is required. Do not check “Diagnostic” or both boxes. If “Diagnostic” is ticked both IgG and IgM testing will be performed. IgM is not needed for the determination of immune status as it is a marker of acute infection.
Hemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.
Preparation Prior to Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Centrifuge if using SST. Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.
Test Frequency and Turnaround Time (TAT)
Mumps immunity serology to detect Mumps IgG is performed daily Monday to Saturday.
Turnaround time is up to 5 days from receipt by PHO laboratory.
Results are reported to the ordering physician or health care provider as indicated on the requisition.
This test is a qualititative test. Quantitative results will ONLY be provided when the reason for testing is for the determination of immunity / immune status and this is clearly indicated on the PHOL requisition. If diagnosis is indicated or ticked quantitative IgG results will not be reported.
The Mumps IgG test is performed using an ELISA (Enzyme Linked Immunosorbent Assay) test kit manufactured by Euroimmun Diagnostics (Germany). This ELISA test provides a quantitative in vitro assay for human antibodies of the IgG class against mumps virus in serum or plasma.
Mumps IgG test results are reported both qualitatively (Non-Reactive, Indeterminate or Reactive) and quantitatively together with an interpretation of the patient’s immune status based on the level of mumps IgG detected.
The unit of mumps IgG antibody measurement is RU/ml (Relative units/millilitre).
Mumps IgG Antibody Ranges and Result Interpretation:
- Non-reactive (<16 RU/ml): No Evidence of Immunity (indicates susceptibility).
- Indeterminate (≥16 - <22 RU/ml): Antibody levels in the indeterminate range for this assay may be insufficient to confer immunity.
- Reactive (≥22 RU/ml): Evidence of past infection / vaccination; evidence of immunity.
These interpretations assume the absence of acute or recent infection.