Salmonella – Enteric culture

Testing Indications

Note to Submitters

Enteric culture is performed at the request of public health units for case management and outbreak investigations. Specimens are accepted only from health units.

If you are a clinician, please use a community laboratory to perform your testing. If you suspect an outbreak contact your local Health Unit.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Salmonella culture1


2.0 ml2,3

Cary Blair

Available in either PHO Cary Blair Kit – Kit Order #390049 or Enteric Bacteriology kit #390036

Salmonella Typhi culture urine only1

Urine (for Salmonella Typhi only)

2.0 ml2,3 of midstream urine

Sterile container without preservative for urine specimen only

Submission and Collection Notes


Enter the name of the organism if you are suspecting a specific organism, e.g. Salmonella


Collect a minimum of 2. 0 ml of liquid faeces or add faeces up to the fill line indicated. DO NOT overfill. Collect a minimum of 2. 0 ml of midstream urine.


The stool specimen must be received in the laboratory and processed within 5 days of collection. Specimens received after 5 days of collection will not be tested. Only one sample per patient is tested per collection date.

The urine specimen must be received and processed within 24 hours of collection. Specimens received after 24 hours of collection will not be tested. Only one sample per patient is tested per collection date.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Salmonella enteric culture testing is performed Monday to Friday.

Turnaround time is up to 6 days from receipt at PHO laboratory.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Specimens that are positive for Salmonella are reported to the Medical Officer of Health as per the Ontario Health Protection and Promotion Act.

Test Methods

Specimens are tested by conventional bacterial culture. 
Bacterial identification includes biotyping and serotyping procedures.

Antimicrobial susceptibility testing is routinely performed on Salmonella Typhi and Salmonella Paratyphi only and for clinical indications (from blood or sterile sites, infants <1 year old, immuno-compromised patients and hospitalized patients–if documented).


Additional Information

For outbreaks, contact your local Health Unit, your local Public Health Laboratory or the Public Health Ontario, Public Health Laboratory Customer Service at416-235-6556 or1-877-604-4567.

contact lab

Contact Laboratory Customer Service

Laboratory Services

Updated 26 March 2019