Pure viable subculture of organism on appropriate media or a swab of the pure subculture in Amies transport medium of the isolate for identification/typing. Note: if a swab is received the TAT will be delayed 24 hours. NB: Primary cultures are unacceptable as they should be processed in the originating lab. The submitter will be contacted by telephone. A report will be issued to indicate that the test has been rejected.
To order PHOL Supplies complete the Requisition for Containers and Supplies. The form should be faxed to Public Health Ontario Laboratory, Toronto at 416-235-5753 or your local PHOL.
Complete the Reference Bacteriology Requisition. Indicate request for typing if required. Please be sure to complete all fields on the requisition, including:• Test Requested• Culture information • Clinical/epidemiology
Place specimen in biohazard bag and seal. Label the specimen with 2 identifiers prior to shipment. Specimen should be transferred to the laboratory within 48 hours of collection.
Outbreak information is required if appropriate.
Streptococcus pyogenes isolates are identified by conventional biochemicals. Isolates from invasive infections as well as outbreak specimens are forwarded to the Public Health Agency of Canada, Winnipeg for emm typing.
Streptococcus pyogenes cultures are tested daily. TAT for typing is up to 21 days.
Results are reported to the ordering physician or health care provider as indicated on the requisition.Specimens that are positive for invasive Streptococcus pyogenes Lancefield group A are to be reported as per Health Protection and Promotion Act.