Antimicrobial Susceptibility Testing – Anaerobes

Conformément au Règlement de l’Ontario 671/92 de la Loi sur les services en français, les renseignements d’analyses de laboratoire liés à la présente page ne sont offerts qu’en anglais parce qu’ils sont de nature scientifique ou technique et destinés uniquement à l’usage des fournisseurs de soins de santé qualifiés et non aux membres du public.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Antimicrobial susceptibility testing – anaerobes

Anaerobic susceptibility test are performed on isolates from sterile sites. Pure viable subculture of 24 – 48 hours growth of organism on  appropriate pre-reduced medium submitted in an anaerobic transport kit or anaerobic swab2,3


Submission and Collection Notes


Complete all fields on the Reference Bacteriology Requisition, including:

  • Test(s) requests
  • Culture information
  • Clinical/epidemiology
  • Date of primary specimen collection
  • Primary source of isolation (mandatory)
  • Contact information

If a swab or broth is received, the turnaround time will be delayed by at least 24 hours.


Primary cultures are unacceptable, and will be rejected. They should be processed in the originating lab.


Mixed or non-viable cultures will not be tested. The submitter will be contacted by telephone. A written report will be issued to indicate that the test has been rejected.

Susceptibility testing on multiple isolates from the same site of infection will not be performed.

Storage and Transport

Label the specimen with the patient’s full name, date of primary specimen collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Complete all fields of the Reference Bacteriology Requisition. Include the patient’s full name, date of birth, Health Card Number (must match the specimen label). Enter the test requested, culture information, clinical/epidemiology information, date of primary specimen collection, primary source of isolation (mandatory), submitting laboratory name and address and clinical diagnosis.

Place in a biohazard bag and seal.

Special Instructions

Transport a fresh subculture in anaerobic conditions to ensure viability on receipt.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Antimicrobial susceptibility testing is performed from Monday to Saturday.

Turnaround time for test results is up to 8 days from date of receipt at the PHO laboratory.

Test Methods

Antimicrobial Susceptibility Testing for anaerobes is performed by E-Test.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Mis à jour le 25 sept. 2019