Fungus Culture – Cerebrospinal Fluid

Conformément au Règlement de l’Ontario 671/92 de la Loi sur les services en français, les renseignements d’analyses de laboratoire liés à la présente page ne sont offerts qu’en anglais parce qu’ils sont de nature scientifique ou technique et destinés uniquement à l’usage des fournisseurs de soins de santé qualifiés et non aux membres du public.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Fungus culture

CSF (Cerebrospinal Fluid)

1.0 ml

Sterile container

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Place specimen in a biohazard bag and seal. Please notify the laboratory if urgent samples are being sent.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Fungus culture is set up daily Monday to Friday.

Turnaround Time for microscopy: 1 day from receipt by PHO laboratory

Turnaround Time for culture: negative cultures are held for up to 28 days

Identification of isolates: dependent on organism(s)

Test Methods

Fungus culture and microscopy:

  • Fungal culture: Identification uses various methods, including microscopic morphology, biochemical reactions, and growth temperature and culture characteristics. Molecular methods will be used when needed.
  • Microscopy uses Calcofluor White Stain. 
  • Full identification will be performed on all isolates.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Mis à jour le 20 juill. 2020