Fungus Culture – Eye

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Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Fungus culture

Biopsy, corneal scrapings, enucleation, ulcers, keratitis, intraocular fluid, vitreous/aqueos eye fluid, lacrimal gland/canal, anterior chamber fluid, washing fluid

Sterile Container

Submission and Collection Notes


Indicate right or left eye and relevant clinical information on the General Test Requisition Form.


Swabs are not suitable specimen.

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Place specimen in a biohazard bag and seal. Store specimen at room temperature. Specimens should be shipped to the PHO laboratory ASAP where possible.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

The Fungus Culture test is set up daily Monday to Friday.

Turnaround Time for microscopy: 1 day

Turnaround Time for culture: up to 28 days

Test Methods

Fungus culture and microscopy:

  • Fungal Culture - for filamentous fungi and yeast. 
  • Microscopy will be done if there is sufficient specimen received. 
  • Microscopy - Calcofluor white 
  • Identification of fungal isolates from culture is performed using various methods including microscopic morphology, biochemical reactions, and growth temperature and cultural characteristics. Molecular methods will be used when needed.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Mis à jour le 20 juill. 2020