
Hepatitis B – Serology Test Ordering Instructions
All sections of the General Test Requisition must be completed. Testing may be delayed or cancelled if clinical information is not provided.
Refer to Hepatitis B Virus Serology Test Information Sheet for detailed testing indications, specimens, methods and reports information.
Test(s) Requested on Requisition | Tests Performed | Notes | Requisition Demonstration |
---|---|---|---|
Hepatitis Serology: Chronic Infection | HBsAg, HBcAb Total (IgG+IgM) |
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Hepatitis Serology: Acute Infection | HBsAg, HBcAb Total (IgG+IgM) |
*HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive | ![]() |
Hepatitis Serology: Immune Status | Anti-HBs | ![]() |
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Hepatitis Serology: Chronic and Immunity Test(s) Requested: Pre-chemotherapy screening |
HBsAg, HBcAb Total (IgG+IgM), Anti-HBs |
For cancer patients prior to commencing systemic therapy (chemotherapy, targeted therapy, immunotherapy) | ![]() ![]() |
Hepatitis Serology: Chronic and Immunity | HBsAg, HBcAb Total (IgG+IgM), Anti-HBs |
A reason for test must be indicated e.g. Dialysis | ![]() ![]() |
Hepatitis Serology: Acute and Immunity | HBsAg, HBcAb Total (IgG+IgM), Anti-HBs |
A reason for test must be indicated e.g. Dialysis *HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive |
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Hepatitis Serology: Chronic Infection and Test(s) Requested: HBeAg, HBeAb |
HBsAg HBcAb Total (IgG+IgM) |
Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive | ![]() ![]() |
Hepatitis Serology: Acute Infection and Test(s) Requested: HBeAg, HBeAb |
HBsAg HBcAb Total (IgG+IgM) |
Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive | ![]() ![]() |
Test(s) Requested: HBsAg, Anti-HBS |
HBsAg Anti-HBS |
Do not complete the Hepatitis Serology section. Under ‘Reason for Test’, select ‘Other’ and specify Hepatitis B Exposure |
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Test(s) Requested: HBeAg and HBeAb or HBcIgM |
HBeAg and HBeAb or HBcIgM |
Do not complete the Hepatitis Serology section. Enter the specific test(s) under Test(s) Requested on the General Test Requisition |
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Needle Stick Injury or Occupational Exposure – Source patient only | HBsAg | Contact Customer Service Center at 416-235-6556/1-877-604-4567 for STAT testing. | ![]() |
Needle Stick Injury or Occupational Exposure – Exposed patient only | HBsAg HBsAb |
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Updated
13 April 2023
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