Hepatitis B – Serology Test Ordering Instructions

All sections of the General Test Requisition must be completed. Testing may be delayed or cancelled if clinical information is not provided.

Refer to Hepatitis B Virus Serology Test Information Sheet for detailed testing indications, specimens, methods and reports information.

Requisition selection(s)	Tests Performed	Notes
Hepatitis Serology: Chronic Infection	HBsAg, HBcAb Total (IgG+IgM)
Hepatitis Serology: Acute Infection	HBsAg, HBcAb Total (IgG+IgM)	*HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
Hepatitis Serology: Immune Status	Anti-HBs
Hepatitis Serology: Chronic and Immunity Test(s) Requested: Pre-chemotherapy screening	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	For cancer patients prior to commencing systemic therapy (chemotherapy, targeted therapy, immunotherapy)
Hepatitis Serology: Chronic and Immunity	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	A reason for test must be indicated e.g. Dialysis
Hepatitis Serology: Acute and Immunity	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	A reason for test must be indicated e.g. Dialysis *HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
Hepatitis Serology: Chronic Infection and Test(s) Requested: HBeAg, HBeAb	HBsAg HBcAb Total (IgG+IgM)	Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive
Hepatitis Serology: Acute Infection and Test(s) Requested: HBeAg, HBeAb	HBsAg HBcAb Total (IgG+IgM)	Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive
Test(s) Requested: HBsAg, Anti-HBS	HBsAg Anti-HBS	Do not complete the Hepatitis Serology section. Under ‘Reason for Test’, select ‘Other’ and specify Hepatitis B Exposure
Test(s) Requested: HBeAg and HBeAb or HBcIgM	HBeAg and HBeAb or HBcIgM	Do not complete the Hepatitis Serology section. Enter the specific test(s) under Test(s) Requested on the General Test Requisition