Hepatitis B – Serology Test Ordering Instructions

All sections of the General Test Requisition must be completed. Testing may be delayed or cancelled if clinical information is not provided.

Refer to Hepatitis B Virus Serology Test Information Sheet for detailed testing indications, specimens, methods and reports information.

Test(s) Requested on Requisition	Tests Performed	Notes
Hepatitis Serology: Chronic Infection	HBsAg, HBcAb Total (IgG+IgM)
Hepatitis Serology: Acute Infection	HBsAg, HBcAb Total (IgG+IgM)	*HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
Hepatitis Serology: Immune Status	Anti-HBs
Hepatitis Serology: Chronic and Immunity Test(s) Requested: Pre-chemotherapy screening	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	For cancer patients prior to commencing systemic therapy (chemotherapy, targeted therapy, immunotherapy)
Hepatitis Serology: Chronic and Immunity	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	A reason for test must be indicated e.g. Dialysis
Hepatitis Serology: Acute and Immunity	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	A reason for test must be indicated e.g. Dialysis *HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
Hepatitis Serology: Chronic Infection and Test(s) Requested: HBeAg, HBeAb	HBsAg HBcAb Total (IgG+IgM)	Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive
Hepatitis Serology: Acute Infection and Test(s) Requested: HBeAg, HBeAb	HBsAg HBcAb Total (IgG+IgM)	Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive
Test(s) Requested: HBsAg, Anti-HBS	HBsAg Anti-HBS	Do not complete the Hepatitis Serology section. Under ‘Reason for Test’, select ‘Other’ and specify Hepatitis B Exposure
Test(s) Requested: HBeAg and HBeAb or HBcIgM	HBeAg and HBeAb or HBcIgM	Do not complete the Hepatitis Serology section. Enter the specific test(s) under Test(s) Requested on the General Test Requisition
Needle Stick Injury or Occupational Exposure – Source patient only	HBsAg	Contact Customer Service Center at 416-235-6556/1-877-604-4567 for STAT testing.
Needle Stick Injury or Occupational Exposure – Exposed patient only	HBsAg HBsAb