Hepatitis B – Serology Test Ordering Instructions

All sections of the General Test Requisition must be completed. Testing may be delayed or cancelled if clinical information is not provided.

Refer to Hepatitis B Virus Serology Test Information Sheet for detailed testing indications, specimens, methods and reports information.

Test(s) Requested on Requisition Tests Performed Notes Requisition Demonstration
Hepatitis Serology: Chronic Infection HBsAg,
HBcAb Total (IgG+IgM)

Hepatitis Serology: Chronic Infection
Hepatitis Serology: Acute Infection HBsAg,
HBcAb Total (IgG+IgM)
*HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive Hepatitis Serology: Acute Infection
Hepatitis Serology: Immune Status Anti-HBs
Hepatitis Serology: Immune Status

Hepatitis Serology: Chronic and Immunity

Test(s) Requested: Pre-chemotherapy screening

HBsAg,
HBcAb Total (IgG+IgM),
Anti-HBs
For cancer patients prior to commencing systemic therapy (chemotherapy, targeted therapy, immunotherapy) Hepatitis Serology: Chronic and Immunity   Test(s) Requested: Pre-chemotherapy screeningHepatitis Serology: Chronic and Immunity   Test(s) Requested: Pre-chemotherapy screening part 2
Hepatitis Serology: Chronic and Immunity HBsAg,
HBcAb Total (IgG+IgM),
Anti-HBs
A reason for test must be indicated e.g. Dialysis Hepatitis Serology: Chronic and Immunity Hepatitis Serology: Chronic and Immunity part 2
Hepatitis Serology: Acute and Immunity HBsAg,
HBcAb Total (IgG+IgM),
Anti-HBs
A reason for test must be indicated e.g. Dialysis
*HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
6.1 Acute Infection and Immune Status 6.2 Acute Infection and Immune Status

Hepatitis Serology: Chronic Infection and

Test(s) Requested: HBeAg, HBeAb

HBsAg
HBcAb Total (IgG+IgM)
Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive 7.1 Chronic Infection and HBeAg,Ab 7.2 Chronic Infection and HBeAg,Ab

Hepatitis Serology: Acute Infection and

Test(s) Requested: HBeAg, HBeAb

HBsAg
HBcAb Total (IgG+IgM)
Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive 7.1 Chronic Infection and HBeAg,Ab8.2 Acute Infection and HBeAg,Ab
Test(s) Requested: HBsAg,
Anti-HBS
HBsAg
Anti-HBS
Do not complete the Hepatitis Serology section.
Under ‘Reason for Test’, select ‘Other’ and specify Hepatitis B Exposure
9.1 HBsAg, anti-HBs 9.2 HBsAg, anti-HBs
Test(s) Requested: HBeAg and HBeAb
or
HBcIgM
HBeAg and HBeAb
or
HBcIgM
Do not complete the Hepatitis Serology section.
Enter the specific test(s) under Test(s) Requested on the General Test Requisition
10.1 HBeAg, HBeAb, HBc IgM
Needle Stick Injury or Occupational Exposure – Source patient only HBsAg  Contact Customer Service Center at 416-235-6556/1-877-604-4567 for STAT testing.  11 Needle Stick Source
Needle Stick Injury or Occupational Exposure – Exposed patient only HBsAg
HBsAb
   12 Needle Stick Recipient
Mis à jour le 13 avr. 2023