Methicillin Resistant Staphylococcus aureus (MRSA) – Pulse Field Gel Electrophoresis (PFGE)

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

MRSA PFGE typing1

Pure bacterial culture of pathogen3 (MRSA) in appropriate agar.


Submission and Collection Notes


MRSA PFGE typing is performed for outbreak investigations and for routine surveillance.  


Include the outbreak number and index case if applicable on the requisition.


Include on the requisition the specimen type  from which the pathogen is isolated.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

The MRSA PFGE typing is performed daily Monday to Friday.

Turnaround time is up to 13 days from receipt by the PHO laboratory.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Test Methods

All MRSA submissions are confirmed as such prior to PFGE testing.

Mis à jour le 20 juill. 2020