Amoebic – Serology

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Amoeba

Serum

1.0 ml

Vacuntainer tubes (SST)

Amoeba

Blood, clotted

5.0 ml

Vacuntainer tubes (SST)

Timing of Specimen Collection

An acute (collected early after the onset of symptoms) and a convalescent (collected 2-3 weeks later) may be required for laboratory diagnosis.

Limitations

Haemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Amoebic (Entamoeba histolytica) serology testing is performed once per week.

Turnaround time is up to 10 days from receipt by PHO Laboratory.

Reporting

Results are reported to the ordering physician or health care provider as indicated on the requisition 

Specimens that are positive for Amoebiasis serology are reported to the Medical Officer of Health as per the Health Protection and Promotion Act.

Test Methods

Amoebic (Entamoeba histolytica) serology testing for IgG is performed using a commercial Enzyme Immunoassay (EIA).

Mis à jour le 22 oct. 2019