Rubella – Avidity

Testing Indications

This test is referred to the Public Health Agency of Canada (PHAC) – National Microbiology Laboratory (NML) in Winnipeg by PHO laboratory. 

Submitters must obtain prior approval for testing from NML prior to specimen collection: TEL: +1 (204)-789-6085 or +1 (204)-789-6080.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Rubella avidity


1.0 ml

Vacutainer tubes (SST)

Rubella avidity


1.0 ml

Lavender top tube

Submission and Collection Notes


Indicate the following on the General Test Requisition Form:

  • pregnancy- specify the number of weeks gestation
  • date of rash and/ fever onset
  • history of vaccination
  • travel/exposure history
  • approval status by NML


Hemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Centrifuge if using SST. Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHO laboratory on ice packs as soon as possible.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Rubella IgG – Avidity testing is referred to the National Microbiology Laboratory (NML) in Winnipeg.

Turnaround time for test results is up to 14 days from receipt by PHO laboratory.


Rubella IgG – Avidity testing is performed using an Enzyme Linked Immunosorbent Assay (ELISA) which determines the avidity of IgG class antibodies against Rubella virus in human serum or plasma.

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Updated 20 July 2020