Test Information Index

Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.

This page provides a directory of resources that provide comprehensive instructions related to laboratory tests performed by Public Health Ontario. This information is for use by qualified health care providers only.

Click on an item below to access testing information including: testing indications, specimen collection and handling, requisitions and kit ordering, special requirements, test methods, turnaround times and reporting.

Acanthamoeba
Acute Severe Hepatitis of Unknown Origin in Children — see Hepatitis (Acute) – Unknown Origin in Children
Adenovirus – Enteric — see Gastroenteritis –Stool Viruses
Adenovirus-Respiratory and Conjunctival — see Respiratory Viruses (including influenza)
Aerobic Colony Count (ACC) – Food — see Food Testing
Aeromonadales – Isolate Identification, Susceptibility, and Typing
Aeromonas – Enteric culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Aluminum
Amoebic – Serology
Anaplasma (Anaplasma phagocytophilum) – Serology
Angiostrongylus
Anisakis
Antimicrobial Susceptibility Testing – Aerobic Bacteria
Antimicrobial Susceptibility Testing – Anaerobes
Arthropods (e.g. Mites, Lice, Fleas, Maggots, and Ticks) – Microscopy
Ascaris – Faeces
Ascaris – Serology — see Ascaris – Faeces
Astrovirus – Enteric
Avian Influenza – Real-time PCR

Babesia – Microscopy and PCR
Babesia microti - Serology
Bacillus anthracis – Confirmation
Bacillus cereus group – Food — see Food Testing
Bacteria – 16S rDNA Test
Bacterial cultures – Aerobic – Reference ID/Confirmation
Bacterial cultures – Anaerobic – Reference Identification/Confirmation
Balantidium coli – Faeces
Bartonella henselae
Bartonella species – PCR
Baylisascaris – Serology
Beach Water — see Public Beach Water
Bordetella - Respiratory - PCR
Bordetella – Serology — see Bordetella - Respiratory - PCR
Bottled Water — see Drinking Water Testing – Official Agencies
Brucella – Serology
Brucella species (B. abortus, B.melitensis, B. suis) – Confirmation
Burkholderia mallei and Burkholderia pseudomallei – Confirmation

Calicivirus (Norovirus, Sapovirus) – Enteric — see Gastroenteritis –Stool Viruses
California Serogroup Serology and PCR
Campylobacter – Enteric Culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Campylobacter spp. – Food — see Food Testing
Campylobacterales – Isolate Identification, Susceptibility, and Typing
Candida auris — see Mycology – Candida auris
Canine Influenza A Virus
Chikungunya Virus- Serology and PCR
Chlamydia pneumoniae – Respiratory PCR
Chlamydia trachomatis – Culture
Chlamydia trachomatis – Lymphogranuloma venereum (LGV) PCR
Chlamydia trachomatis – Lymphogranuloma venereum (LGV) Serology — see Chlamydia trachomatis – Lymphogranuloma venereum (LGV) PCR
Chlamydia trachomatis/Neisseria gonorrhoeae (CT/GC) – Nucleic Acid Amplification Testing (NAAT) – Urine — see Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) – Nucleic Acid Amplification Testing (NAAT)
Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) – Nucleic Acid Amplification Testing (NAAT)
Chlamydophila psittaci – Polymerase Chain Reaction (PCR)
Chlamydophila psittaci – Serology — see Chlamydophila psittaci – Polymerase Chain Reaction (PCR)
Clonorchis sinensis
Clostridioides difficile – Antigen, PCR, Susceptibility, and Typing
Clostridioides difficile – Culture and Pulse Field Gel Electrophoresis (PFGE) Typing — see Clostridioides difficile – Antigen, PCR, Susceptibility, and Typing
Clostridium perfringens – Food — see Food Testing
Clostridium perfringens (Stool) – Antigen
Coliform – Food — see Food Testing
Coliform – Official Agency Drinking Water — see Drinking Water Testing – Official Agencies
Coliform – Private Citizen Drinking Water — see Drinking Water Testing – Private Citizen
Coronavirus Disease 2019 (COVID-19) – PCR
Coronavirus Disease 2019 (COVID-19) - Variant of Concern Screening and Whole Genome Sequencing Surveillance
Coxsackie — see Enterovirus
CPE – Carbapenemase Producing Enterobacterales – Confirmation
Creutzfeldt-Jakob Disease (CJD)
Cryptococcal Antigen Latex Agglutination – Direct Blood and CSF
Cryptosporidium – Faeces
Cyclospora – Faeces
Cyclospora - Subtyping
Cysticercosis – Serology
Cystoisospora belli (syn. Isospora belli) – Faeces
Cytomegalovirus – Serology including Avidity
Cytomegalovirus Detection – Real Time PCR

Demodex folliculorum — see Arthropods (e.g. Mites, Lice, Fleas, Maggots, and Ticks) – Microscopy
Dengue Virus - Serology and PCR
Dientamoeba fragilis – Faeces
Diphtheria – Culture, Reference Isolates Identification Confirmation & Toxin Production Confirmation
Diphtheria – Serology
Diphyllobothrium species
Drinking Water Testing – Official Agencies
Drinking Water Testing – Private Citizen

E. coli – Official Agency Drinking Water — see Drinking Water Testing – Official Agencies
E. coli – Private Citizen Drinking Water — see Drinking Water Testing – Private Citizen
E. coli – Suspected Sewage Contamination – Water, Sewage — see Sewage
E. coli O157 and Non-O157 Shiga Toxin Producing E. coli (STEC) – Enteric Culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Eastern Equine Encephalitis Virus – Serology and PCR
Ebola — see Viral Haemorrhagic Fevers – Including Ebola Disease
Echinococcosis – Serology
Echinococcus – Cyst
Echovirus — see Enterovirus
Ehrlichia chaffeensis – Serology
Entamoeba histolytica – Abscess Liver
Entamoeba histolytica – Faeces-Para
Entamoeba histolytica/dispar – Differentiation
Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Enterobacterales – Isolate Identification, Susceptibility, and Typing
Enterobius vermicularis – Faeces
Enterovirus
Epstein Barr Virus (EBV) – Serology
ESBL - Extended Spectrum Beta-Lactamase Confirmation
Escherichia coli – Food — see Food Testing
Escherichia coli O157:H7 – Food — see Food Testing

Fasciola – Faeces
Fasciola – Serology
Filaria – Microscopy
Filaria Antibody – Serology
Food Testing
Francisella tularensis – Confirmation
Francisella tularensis – Serology
Fungal – Serology

GAS – Group A Streptococcus – Outbreak — see Streptococcus pyogenes (Group A Streptococcal Disease or GAS) – Typing
GAS – Group A Streptococcus – Serotyping — see Streptococcus pyogenes (Group A Streptococcal Disease or GAS) – Typing
Gastroenteritis –Stool Viruses
Giardia intestinalis (syn. Giardia lamblia, G. duodenalis)- Faeces
Gnathostoma – Serology — see Worm Identification
Group A Streptococci (GAS) – Pulse Field Gel Electrophoresis (PFGE) — see Streptococcus pyogenes (Group A Streptococcal Disease or GAS) – Typing

Haemophilus ducreyi – Polymerase Chain Reaction (PCR)
Haemophilus influenzae – Confirmation and Serotyping
Hantavirus - Serology and PCR
HCV Drug Resistance
Helicobacter pylori – Antibody (IgG)
Hepatitis (Acute) – Unknown Origin in Children
Hepatitis A – Genotyping-Subtyping
Hepatitis A – Serology
Hepatitis B – Autopsy
Hepatitis B - DNA Viral Load
Hepatitis B – Genotyping
Hepatitis B – Serology
Hepatitis C – Autopsy
Hepatitis C – Diagnostic Serology
Hepatitis C – Genotyping/Subtyping
Hepatitis C - RNA Viral Load
Hepatitis C – Supplemental Testing — see Hepatitis C – Diagnostic Serology
Hepatitis D – Serology
Hepatitis E – Serology
Herpes B Virus – Serology and PCR
Herpes Simplex (HSV) and Varicella Zoster (VZV) Viruses Molecular testing
Herpes simplex virus 1 & 2 – Serology
Heterophyes – Faeces
Heterotrophic Plate Count – Official Agency Drinking Water — see Drinking Water Testing – Official Agencies
HIV – Autopsy
HIV – Diagnostic Serology
HIV – PCR
HIV Genotyping, Resistance, Tropism and HLA-B*57:01 Abacavir Hypersensitivity Testing
HIV-1 RNA Viral Load
Hookworm (Ancylostoma duodenale/Necator americanus) – Faeces
HTLV I and II – Serology
Human Metapneumovirus – Respiratory — see Respiratory Viruses (including influenza)
Human Papilloma Virus (HPV) – Genotyping
Hymenolepis – Faeces

Ice – Treated
Influenza – Avian – Real-time PCR — see Avian Influenza – Real-time PCR
Influenza – Respiratory — see Respiratory Viruses (including influenza)
Influenza Subtyping — see Avian Influenza – Real-time PCR
Isospora - Faeces — see Cystoisospora belli (syn. Isospora belli) – Faeces

Japanese Encephalitis Virus – Serology and PCR

Lead – Blood and Urine Testing
Legionella – Respiratory–PCR and Culture
Legionella – Urine antigen
Legionella spp. – Swab
Legionella spp. – Water
Leishmania – Microscopy and PCR
Leishmania – Serology
Leptospira – Serology and PCR
Lice – ID — see Arthropods (e.g. Mites, Lice, Fleas, Maggots, and Ticks) – Microscopy
Listeria – Identification and Subtyping
Listeria monocytogenes – Food — see Food Testing
Lyme Disease - PCR
Lyme Disease – Serology
Lymphocytic Choriomeningitis – Serology and PCR

Maggots – Myiasis — see Arthropods (e.g. Mites, Lice, Fleas, Maggots, and Ticks) – Microscopy
Malaria (Plasmodium) – Microscopy, RDT, PCR and Typing
Malaria (Plasmodium) – Serology
Measles – Diagnostic – PCR
Measles – Serology
Methicillin Resistant Staphylococcus aureus (MRSA) – Outbreak — see MRSA – Methicillin Resistant Staphylococcus aureus – Confirmation
Methicillin Resistant Staphylococcus aureus (MRSA) – Pulse Field Gel Electrophoresis (PFGE)
Metorchis – Faeces
Microsporidia – Biopsy
Microsporidia – Faeces/Bowel Aspirate
Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
Molluscum contagiosum virus detection
Monkeypox Virus
MRSA – Methicillin Resistant Staphylococcus aureus – Confirmation
Mumps – Diagnostic – PCR
Mumps – Diagnostic Serology — see Mumps – Serology
Mumps – Immunity Serology — see Mumps – Serology
Mumps – Serology
Mycobacterium – Blood — see Mycobacterium – Culture
Mycobacterium – Corneal Scrapings — see Mycobacterium – Culture
Mycobacterium – CSF, Fluids, Bone or Tissue — see Mycobacterium – Culture
Mycobacterium – Culture
Mycobacterium – Faeces — see Mycobacterium – Culture
Mycobacterium – Gastric Lavage, — see Mycobacterium – Culture
Mycobacterium – MTBC and Rifampin/Isoniazid Resistance PCR
Mycobacterium – NTM-Susceptibility
Mycobacterium – Referred Cultures — see Mycobacterium – Culture
Mycobacterium – Respiratory — see Mycobacterium – Culture
Mycobacterium – Superficial Abscess, Lesion, Wound or Aspirate — see Mycobacterium – Culture
Mycobacterium – TB Susceptibility Testing
Mycobacterium – Urine — see Mycobacterium – Culture
Mycobacterium abscessus – Molecular Subspecies Identification and Macrolide Resistance Testing
Mycobacterium leprae – PCR
Mycology – Candida auris
Mycology – Fungal Culture – Superficial/Dermatophytes
Mycology - Fungal Culture - Systemic
Mycology – Nocardia and Aerobic Actinomycetes Culture
Mycology – Pneumocystis jirovecii pneumonia (PJP) Detection
Mycology – Reference Identification of Yeast, Filamentous Fungi and Nocardia/Aerobic Actinomycetes
Mycology – Susceptibility Testing of Nocardia and Aerobic Actinomycetes
Mycology – Susceptibility Testing of Yeast and Filamentous Fungi
Mycoplasma – urogenital — see Ureaplasma/Mycoplasma – Culture and Reference Identification
Mycoplasma genitalium — see Ureaplasma/Mycoplasma – Culture and Reference Identification
Mycoplasma pneumonia – Serology
Mycoplasma pneumoniae – Respiratory PCR

Neisseria gonorrhoeae – Culture, Reference Identification and Susceptibility
Neisseria gonorrhoeae and Chlamydia trachomatis – NAAT — see Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) – Nucleic Acid Amplification Testing (NAAT)
Neisseria meningitidis – Identification, Confirmation and Serogrouping
Neisseria meningitidis – Real-time PCR
Norovirus – Enteric — see Gastroenteritis –Stool Viruses
Novel Coronavirus (2019-nCoV) — see Coronavirus Disease 2019 (COVID-19) – PCR

Onchocerca – Nodule – Worm
Orientia tsutsugamushi (Scrub Typhus)
Oropouche Virus

Paragonimus – Serology
Paragonimus westermani
Parainfluenza virus – Respiratory — see Respiratory Viruses (including influenza)
Parasite – Bowel
Parasite – Faeces
Parechovirus – PCR and Genotyping
Parvovirus – Serology
Pertussis — see Bordetella - Respiratory - PCR
pH – Food — see Food Testing
Phosphatase – Food — see Food Testing
Pinworm – Enterobius vermicularis
Poliovirus – Isolation and Characterization
Polyomavirus – BK/JC Virus
Pool Water — see Recreational Water Facilities and Public Pools Spas
Powassan Virus – Serology and PCR
Poxvirus - Skin
Prenatal – Serology
Public Beach Water

Q Fever – Serology

Rabies – Serology
Recreational Water – Public Beach — see Public Beach Water
Recreational Water Facilities — see Recreational Water Facilities and Public Pools Spas
Recreational Water Facilities and Public Pools Spas
Respiratory Viruses (including influenza)
Rhinovirus — see Respiratory Viruses (including influenza)
Rickettsia (Spotted Fever Group and Typhus Group)
Ross River Virus
Rotavirus – Enteric — see Gastroenteritis –Stool Viruses
RSV – Culture Direct — see Respiratory Viruses (including influenza)
Rubella – Avidity
Rubella – Diagnostic (Molecular Testing by RT–PCR)
Rubella – Diagnostic Serology — see Rubella – Serology
Rubella – Immunity Serology — see Rubella – Serology
Rubella – Serology

Salmonella – Enteric culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Salmonella spp. – Food — see Food Testing
Sapovirus – Enteric — see Gastroenteritis –Stool Viruses
Sarcocystis – Faeces
SARS – RT-PCR
Scabies (Sarcoptes scabiei) – Scrapings — see Arthropods (e.g. Mites, Lice, Fleas, Maggots, and Ticks) – Microscopy
Schistosoma – Serology
Schistosoma haematobium – Urine
Schistosoma spp. – Faeces
Sewage
Shigella – Enteric culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Shigella spp. – Food — see Food Testing
Slide Examination – Genital Smear
Spa — see Recreational Water Facilities and Public Pools Spas
St. Louis Encephalitis Virus
Staphylococcus aureus – Food — see Food Testing
Staphylococcus aureus Enterotoxin – Food — see Food Testing
Streptococcus agalactiae – Serotyping
Streptococcus pneumoniae – RT-PCR
Streptococcus pneumoniae – Serotyping
Streptococcus pyogenes (Group A Streptococcal Disease or GAS) – Typing
Strongyloides – Serology
Strongyloides stercoralis – Faeces/other Effluents
Suspicious Packages (for Investigation of Agents of) Bioterrorism – RT PCR
Syphilis – Chancre – Direct Fluorescence
Syphilis – CSF
Syphilis – Serology

Taenia – Faeces
Tetanus – Serology
Tick Identification – Surveillance — see Arthropods (e.g. Mites, Lice, Fleas, Maggots, and Ticks) – Microscopy
Tick-borne encephalitis (TBE) Serology and PCR– Referred Out
Torovirus – Enteric — see Gastroenteritis –Stool Viruses
Total Gram Negative Count – Food — see Food Testing
Toxocara – Serology
Toxoplasma – Microscopy and PCR
Toxoplasma – Serology
Trichinella – Biopsy
Trichinella – Serology
Trypanosoma – Microscopy and PCR
Trypanosoma American or African – Serology
Tularemia (Francisella tularensis) Serology

Ureaplasma/Mycoplasma – Culture and Reference Identification

Vancomycin Resistant Enterococci (VRE) – Pulse Field Gel Electrophoresis
Vancomycin Resistant Enterococcus (VRE) – Confirmation
Varicella – Serology
Varicella Zoster Virus Detection — see Herpes Simplex (HSV) and Varicella Zoster (VZV) Viruses Molecular testing
Venezuelan Equine Encephalitis Virus
Vibironales – Isolate Identification, Susceptibility, and Typing
Vibrio – Enteric culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Vibrio spp. – Food — see Food Testing
Viral Haemorrhagic Fevers – Including Ebola Disease
Virus Detection – CSF
Virus Detection – Tissue
VRE – Vancomycin Resistant Enterococci – Outbreak — see Vancomycin Resistant Enterococcus (VRE) – Confirmation

Water – Private Citizen Drinking Water — see Drinking Water Testing – Private Citizen
Water – Suspected Sewage Contamination — see Sewage
Water Activity – Food — see Food Testing
West Nile Virus – Serology and PCR
Western Equine Encephalitis Virus – Serology and PCR
Whipples disease – PCR
Whooping Cough — see Bordetella - Respiratory - PCR
Worm Identification

Yeast and Mould – Food — see Food Testing
Yellow Fever Virus
Yersinia – Enteric culture — see Enteric Bacteria (including Salmonella, Shigella, STEC, Plesiomonas, Yersinia, Aeromonas, Vibrio, or Campylobacter) – Culture and PCR
Yersinia – Food — see Food Testing
Yersinia – Serology
Yersinia pestis

Zika Virus - Serology and PCR
Zinc Protoporphyrin – Blood testing