Neisseria meningitidis – Real-time PCR

Testing Indications

This test is offered under the following conditions:

  • When the clinical picture is consistent with a diagnosis of invasive meningococcal disease. 
  • When the submitting laboratory is unable to culture N. meningitidis after 24-48 hours incubation and meningococcal disease is still suspected (e.g. due to use of antibiotics prior to obtaining a specimen from the patient)

Contact PHO Laboratory Customer Service Centre prior to collection of specimens

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Neisseria meningitidis PCR

CSF

250 μl

Sterile container

Neisseria meningitidis PCR

Whole blood or plasma in EDTA

250 μl

ETDA

Submission and Collection Notes

1

Include gram stain results and clinical information on the  General Test Requisition Form.

Limitations

Haemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

The Neisseria meningitidis – Real-time PCR test is performed as needed Monday to Friday. 

Turnaround time is up to 3 days from receipt by PHO laboratory.

STAT and Critical Samples Testing

Testing on weekends may be performed under special circumstances. Contact the After-hours emergency duty officer at 416-605-3113 for after-hours consultation.

Reporting

Results are reported to the ordering physician or health care provider as indicated on the requisition. 

Specimens that are positive for Neisseria meningitidis are to be reported to the Medical Officer of Health as per Health Protection and Promotion Act

Test Methods

Neisseria meningitidis specimen nucleic acids are tested using a Real-Time polymerase chain reaction (PCR) detection of a Neisseria meningitidis carbohydrate transporter (ctrA) and capsular biosynthesis genes for serogroups A, B, C, W135, X and Y. 

The sensitivity of the Real Time PCR test is higher than conventional culture.

contact lab

Contact Laboratory Customer Service

Laboratory Services

customerservicecentre@oahpp.ca

Updated 21 Dec 2018