Bordetella – Respiratory
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
Nasopharyngeal (NP) swab
Sputum (including induced), Tracheal aspirates also acceptable, but NP swab and NP aspirates preferred
Submission and Collection Notes
Timing of Specimen Collection
Specimens should be collected during the acute phase of illness.
The PCR transport medium is colourless. NB: Do not use Multi-organism transport medium which is pink.
Preparation Prior to Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Test Frequency and Turnaround Time (TAT)
Bordetella pertussis testing is performed daily Monday to Friday.
Turnaround time is up to 4 days from receipt by PHO laboratory.
Results are reported to the ordering physician or health care provider as indicated on the requisition.
Bordetella – respiratory specimens are tested by a Polymerase Chain Reaction (PCR) test that detects Bordetella pertussis, Bordetella parapertussis and Bordetella holmesii. The PCR is >99% specific, and more sensitive than routine culture.