Brucella species (B. abortus, B.melitensis, B. suis) – Confirmation

Testing Indications

Submitting laboratories must contact Public Health Ontario laboratory Customer Service Centre by telephone prior to submission of the isolate.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Brucella species (B. abortus, B. melitensis, B.suis) – Confirmation

Pure viable subculture of organism on blood or Columbia sheep blood agar plate.

Most appropriate medium is blood or Columbia sheep blood agar plate.

Submission and Collection Notes


Complete all fields of the General Test Requisition Form, including:

  • test(s) requested
  • culture information (e.g. colonial and gram morphology, urea test if available)
  • clinical/epidemiology
  • contact information

Preparation Prior to Transport

Label the specimen with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Culture must be viable. Use a fresh subculture and ship in conditions to ensure viability on receipt.

Brucella species must be handled in accordance with the Canadian Biosafety Standards and shipped in accordance with the Transportation of Dangerous Goods Regulations.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Suspected culture of Brucella species is forwarded to the National Microbiology Laboratory (NML) Monday to Thursday. 

Turnaround time for test results is up to 3 days from receipt by NML.

STAT and Critical Samples Testing

Urgent testing is available upon request by calling the Customer Service Centre 416-235-6556 or 1-877-604-4567.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Specimens that are positive for Brucella are to be reported to the Medical Officer of Health as per Health Protection and Promotion Act.

Test Methods

Brucella species is identified and confirmed using real-time-PCR (Polymerase Chain Reaction) method.

contact lab

Contact Laboratory Customer Service

Laboratory Services

Updated 25 Sep 2019