Chlamydia pneumoniae – Respiratory PCR

Testing Indications

Health Unit staff should contact the public health laboratory if an outbreak is suspected prior to collection of samples.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Chlamydia pneumoniae

Nasopharyngeal Swab (NPS), or tracheal aspirates, or respiratory tract specimens

Swab in transport media provided in kit

Mycoplasma pneumoniae / Chlamydia pneumoniae PCR Kit; Kit Order# 390085

Submission and Collection Notes


Indicate symptoms and date of onset on the General Test Requisition Form if diagnostic testing is required.

Timing of Specimen Collection

Specimens should be collected as soon as possible following onset of symptoms using the Mycoplasma pneumoniae / Chlamydia pneumoniae collection kit.

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens should be stored at 2-8°C following collection and shipped to PHO laboratory on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Chlamydia pneumoniae testing is performed twice per week Monday to Friday. 

Turnaround time is up to 5 days from receipt by PHO laboratory.

Test Methods

Chlamydia pneumoniae specimens are tested by a PCR – Polymerase Chain Reaction test that detects Mycoplasma pneumoniae and Chlamydia pneumoniae. The PCR is >99% specific.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

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Laboratory Services

Updated 20 July 2020