Leishmania – Serology
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
Leishmania serology, or Leishmaniasis, Kala-azar serology
Blood or serum
5.0 ml blood or 1.0 ml serum
Blood, clotted - vacutainer tubes (SST)
Submission and Collection Notes
Timing of Specimen Collection
An acute (collected early after the onset of symptoms) and a convalescent (collected 2-3 weeks later) may be required for laboratory diagnosis.
Haemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Centrifuge if using SST. Place specimen in biohazard bag and seal.
Test Frequency and Turnaround Time (TAT)
Leishmania serology is a Referred Out Test. Samples are sent to the National Reference Centre for Parasitology (NRCP) in Montreal.
Turnaround time is up to 42 days from receipt by PHO laboratory.
Leishmania serology is performed using Indirect Immunofluorescence (IFA) methodology.