Pneumocystis jirovecii – Pneumocystis pneumonia

Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Pneumocystis jirovecii or Pnuemocystis pneumonia

Bronchial Alveolar Lavages (BAL), or pleural fluid


Sterile container

Pneumocystis jirovecii or Pnuemocystis pneumonia



Sterile container

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Place specimen container in the biohazard bag and seal bag, and insert the completed requisition in the pocket on the outside of the sealed biohazard bag. Specimens should be stored at 2-8°C following collection and shipped to PHO laboratory on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Pneumocystis jirovecii specimen testing is performed by microscopy daily Monday to Friday and on holidays when mycology staff are scheduled.

Turnaround time is 24 hrs for weekdays from receipt by PHO laboratory. For Saturday and holiday testing specimen will be tested on the next available business day.

Test Methods

Specimens for Pneumocystis jirovecii are tested by Fungifluor microscopy.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

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Laboratory Services

Updated 20 July 2020