CPE – Carbapenemase Producing Enterobacteriaceae – Confirmation
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
CPE – Carbapenemase producing Enterobacteriaceae
Pure viable subculture of organism on appropriate medium (Columbia sheep blood agar plate) or a swab of a pure subculture of the organism in amies transport medium
Submission and Collection Notes
Specimen Type: Pure viable subculture of 24 – 48 hours growth of organism on an appropriate medium or a swab for CPE confirmation test.
Note: if a swab or broth is received the turnaround time will be delayed by at least 24 hours.
NB: Primary cultures are unacceptable as they should be processed in the originating lab. The submitter will be contacted by telephone. A report will be issued to indicate that the test has been rejected.
Preparation Prior to Transport
Label the specimen with at least 2 identifiers and place in a biohazard bag and seal.
NB: Culture must be viable. Use a fresh subculture and ship in conditions to ensure viability on receipt.
Test Frequency and Turnaround Time (TAT)
CPE confirmation testing is performed daily.
Turnaround time is up to 3 days.
Results are reported to the ordering physician or health care provider as indicated on the requisition.
CPE confirmation is performed on Enterobacteriaceae using phenotypic inhibitor method and multiplex PCR assay.