Diphtheria culture – Skin lesions
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
Aspirate, Swab or membranous material
Amies Charcoal Transport Media; Kit Order #300229
Submission and Collection Notes
Material should be taken from the affected area of the skin, either by aspirate or swab. Membranous material may also be cultured.
Remove any crusted material and swab the base of the lesion(s).
Indicate symptoms and date of onset on the General Test Requisition Form.
Preparation Prior to Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Specimens should be sent to the laboratory as soon as possible, however swabs are acceptable within 3 days of collection.
Specimens should be stored at 2-8°C following collection and shipped to PHO laboratory on ice packs.
Test Frequency and Turnaround Time (TAT)
Diphtheria culture is performed daily, Monday to Friday.
Turnaround time is up to 5 days from receipt by PHO laboratory.
Results are reported to the ordering physician or health care provider as indicated on the requisition.
Specimens that are positive for Diphtheria are to be reported to the Medical Officer of Health as per Health Protection and Promotion Act.
Specimens are set up on bacterial culture medium for identification and isolates are tested for toxigenicity.