Haemophilus ducreyi – Polymerase Chain Reaction (PCR)

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Haemophilus ducreyi



Genital ulcer swab

1.0 mL of fluid

CHL(C) kit / Chlamydia trachomatis culture kit order # 390083 or a universal transport medium (UTM) for swabs.

Haemophilus ducreyi



Bubo aspirate

1.0 ml

Sterile container

Submission and Collection Notes


Dacron or cotton swabs are required for specimen collection. Collect specimen from the base of the genital ulcer or collect an aspirate of the bubo. Clean area with physiological saline and swab base of genital ulcer. Alternately aspirate bubo and submit in sterile container.


Dry swabs are acceptable for testing.


Patient clinical history must be indicated on the requisition for testing to be performed at the NML. Documentation must include clinical background leading to suspicion of H. ducreyi infection in a patient, including any additional factors that may increase the probability of H. ducreyi infection. Chancroid is endemic in southeast Asia and Africa, but infrequent in the developed world. Travel to endemic areas must be indicated on the requisition.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection, and one other unique identifier such as the patient’s date of birth or Health Card Number.  Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens for PCR/molecular detection can be stored at 2-8°C or frozen. Bubo aspirates should be shipped frozen.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Specimens submitted for Haemophilus ducreyi PCR are shipped every Thursday to the NML for testing.  

Turnaround time is up to 28 days from receipt by PHO laboratory.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Test Methods

Haemophilus ducreyi PCR is a referred out test. Specimens will be shipped to the National Microbiology Laboratory (NML) in Winnipeg.

Refer to external resources – Haemophilus ducreyi Molecular detection by PCR.

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Updated 4 Jan 2019