Mycobacterium – Referred Cultures

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Mycobacterium

Acid Fast Bacillus (AFB)

N/A

N/A

Submission and Collection Notes

1

Submit viable growth of an Acid Fast Bacillus (AFB) on solid or in liquid Mycobacterium culture medium.

2

Contaminated or non-viable cultures will not be tested.

Limitations

Identification assays can only be performed on cultures and not direct from specimens.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Place culture in a biohazard bag and seal.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Speciation of mycobacterial cultures is performed daily Monday to Friday.

Turnaround time is up to 7 days from receipt by PHO laboratory.

Reporting

Results are reported to the submitting laboratory as indicated on the requisition.

All new isolates of Mycobacterium tuberculosis are telephoned to the submitting laboratory within 24 hours of identification.

Specimens that are positive for Mycobacterium tuberculosis are to be reported to the Medical Officer of Health as per Health Protection and Promotion Act.

Test Methods

Mycobacteria are identified by MALDI-TOF, the GenoType Mycobacterium line-probe assay test (Hain Lifescience), MPT64 protein test (SD Bioline) or by sequence analysis.

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Laboratory Services

customerservicecentre@oahpp.ca

Updated 21 Dec 2018