Mycoplasma pneumoniae – Respiratory PCR

Testing Indications

Contact your local Public Health Unit if you suspect an outbreak.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Mycoplasma pneumoniae PCR

Nasopharyngeal Swab (NPS), or tracheal aspirates, or respiratory tract specimens

Swab in transport media provided in kit

Mycoplasma pneumoniae / Chlamydophila pneumoniae PCR Kit Order# 390085

Submission and Collection Notes


Indicate symptoms and date of onset on the General Test Requisition Form if diagnostic testing is required.

Timing of Specimen Collection

Specimens should be collected as soon as possible following onset of symptoms using the Mycoplasma pneumoniae / Chlamydophila pneumoniae collection kit.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens should be collected during the acute phase of illness. Specimens should be stored at 2-8°C following collection and shipped to PHO laboratory on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

The Mycoplasma pneumoniae PCR is tested twice weekly, Monday to Friday.

Turnaround time is up to 5 days from receipt by PHO laboratory.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Test Methods

Mycoplasma pneumoniae specimens are tested by a PCR (Polymerase Chain Reaction) test that detects Mycoplasma pneumoniae and Chlamydophila pneumoniae. The PCR is >99% specific.

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Laboratory Services

Updated 13 Jan 2020