Mycoplasma pneumoniae – Respiratory PCR

Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.

Testing Indications

Mycoplasma pneumoniae is a common cause of upper respiratory tract infections, bronchitis, and “atypical” community-acquired pneumonia. Clinical manifestations outside the respiratory tract are rare but may occur. M. pneumoniae is transmitted via respiratory droplets and can cause sporadic infections and sustained outbreaks.

If an outbreak is suspected, contact your local Public Health Unit.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Mycoplasma pneumoniae PCR

Nasopharyngeal Swab (NPS), or tracheal aspirates, or respiratory tract specimens

Swab in transport media provided in kit

Mycoplasma pneumoniae / Chlamydophila pneumoniae PCR Kit Order# 390085

Submission and Collection Notes


Indicate symptoms and date of onset on the General Test Requisition Form if diagnostic testing is required.

Timing of Specimen Collection

Specimens should be collected as soon as possible following onset of symptoms using the Mycoplasma pneumoniae / Chlamydophila pneumoniae collection kit.

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens should be collected during the acute phase of illness. Specimens should be stored at 2-8°C following collection and shipped to PHO laboratory on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Mycoplasma pneumoniae PCR is tested twice weekly, Monday to Friday.

Turnaround time is up to 5 days from receipt by PHO Laboratory.

Test Methods

Testing for Mycoplasma pneumoniae is performed using a laboratory-developed multiplex real-time polymerase chain reaction (PCR) assay that detects both Mycoplasma pneumoniae and Chlamydia pneumoniae. The assay has >98% sensitivity and 100% specificity for the detection of Mycoplasma pneumoniae.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

contact lab

Contact Laboratory Customer Service

Laboratory Services

Updated 20 July 2020