Fungus Culture – Subcutaneous infections/Tissues
(Sporotrichosis Serology is not available through PHO Public Health Laboratories)
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
Any of: Skin ulcers, biopsy/autopsy materials, surgically collected sinus wall scrapings, tissue
Submission and Collection Notes
If insufficient sample is received, culture only will be done.
For skin ulcers, biopsies are preferable to swabs since the presence of fungal elements within tissues is a diagnosis of invasive disease. The biopsy should be taken near the periphery of the lesion and should include enough tissue for culture. Swabs are discouraged.
Preparation Prior to Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Place specimen in a biohazard bag and seal. Store specimen at room temperature. Specimens should be shipped to the lab ASAP where possible.
Test Frequency and Turnaround Time (TAT)
Fungus culture is set up daily Monday to Friday.
Turnaround Time for microscopy: 1 day from receipt by PHO laboratory
Turnaround Time for culture: up to 28 days (for negative result)
Identification of isolates: dependent on organism(s)
Results are reported to the ordering physician or health care provider as indicated on the requisition.
Fungus culture: includes fungal culture and fungal microscopy.
Specimens are tested by microscopy and culture for dermatophytes, yeast and non-dermatophytic mould pathogens of keratinized tissue.
Microscopy uses calcofluor white Stain.
Fungal culture identification uses various methods, including microscopic morphology, biochemical reactions, and growth temperature and culture characteristics. Molecular methods are used when needed.