Virus Detection – Tissue
Submission and Collection Notes
Molecular methods are preferred over viral culture for detecting viruses in tissue, due to increased sensitivity; please contact PHO for information on availability of molecular testing prior to collecting sample.
Using a sterile container and you are submitting smaller pieces of tissue, add phosphate buffered saline (PBS) to cover the tissue to prevent drying.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHO on ice packs.
Test Frequency and Turnaround Time (TAT)
Virus culture testing is performed Monday to Friday. Turnaround time is up between 2-18 days (see test methods for more detail) from the time of receipt at PHO Laboratory.
Molecular testing is performed Monday to Friday. TAT is up to 3 days from the time of receipt at PHO laboratory.
STAT and Critical Sample Testing is not available.
Samples are observed in culture for a minimum of 16 days to allow for growth of the virus in culture. Samples that are positive can be observed at any point during this period, and can be reported up to 18 days.
Tissue samples may also be tested by molecular methods depending on sample type, patient setting and requested organism.