COVID-19 Saliva Collection Kit –Saliva (Neat) with Funnel

How to Collect a Saliva Sample for a COVID 19 PCR Test

This step-by-step guide shows you how to use a saliva test kit to collect a saliva specimen for a COVID-19 test.

Duration: 3 min

Click here to download a PDF of the COVID-19 Saliva Collection Kit –Saliva (Neat) with Funnel


Before collecting saliva:

  • Please read all of these instructions before beginning
  • Drink a cup of water 60 minutes before collecting saliva to ensure adequate hydration for saliva flow.
  • Do NOT eat, drink, chew gum, smoke, or vape for at least 30 minutes before collecting saliva
  • Wash hands with soap and water or hand sanitizer

For specimen collection centre personnel:

  1. While wearing appropriate personal protective equipment (PPE), open the sealed bag and remove the specimen container and funnel from the package.
  2. Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Saliva collection using funnel method

  1. Make sure you are wearing a face mask.
  2. Wash your hands with soap and water or hand sanitizer
  3. Ensure the collection tube is labelled with your information including name, date of birth, collection date.
  4. Unscrew the cap of the tube/container in the supplied kit. Place the cap upside down in a clean, secure spot as it will be used to seal the sample.
  5. The healthcare provider or you need to push the funnel supplied in the kit into the top of the tube. The funnel should fit snugly into the tube opening.
  6. To promote saliva production rub the inside of your cheek with your tongue or gently massage the outside of your cheek.
  7. Remove your mask
  8. Allow saliva to pool in your mouth. Begin carefully spitting onto the tapered inside of the funnel allowing the saliva to run down into the tube. Try to avoid spitting too much at once or into the center of the funnel.
  9. Continue to spit into the funnel until the level in the tube reaches the fill line, between 2-3 ml not including the bubbles. This may take 2-5 minutes
  10. Remove the funnel and discard in designated waste bin. Put the cap on the collection tube tightly. Wipe away any collection material that may have contacted the outside of the tube.
  11. Return the tube to the specimen collection personnel.
  12. Put on your mask.
  13. Wash your hands with soap and water or hand sanitizer

For specimen collection center personnel:

  1. Ensure cap is on tightly.
  2. Place specimen in the biohazard bag.
  3. Complete all fields of the COVID-19 test requisition: include, the patient’s full name, date of birth, Health Card Number (must match the specimen label), source of specimen, date of onset, date of collection, physician name and address, and clinical diagnosis and place it in the attached outer pocket of the specimen bag, so it is not exposed to the specimen.
  4. To maintain optimum viability, the specimen should be stored and transported at 2-8°C. If transport to the laboratory will be delayed, specimen should be frozen at -70°C or below and shipped on dry ice.

To order kits or for more information: Submit your order via the Ontario Health Digital Services page

Updated 29 Oct 2020