Laboratory Contact

We undertake important tests for Ontario’s public health units, clinicians in primary care, hospitals, and long-term care facilities, as well as conducting reference testing for partner laboratories across the province. Below you will find contact information for our laboratories located across the province. Clinicians and Public Health Units can contact Customer Service for help with:

Public Health Ontario also undertakes testing for private drinking water system owners (e.g., well water, treated lake water, water from cisterns). Private drinking water test results are available by phone, in person or by mail.

Laboratory Hours of Operation

PHO’s laboratory conducts testing seven days a week.

Laboratory Customer Service

(Monday–Friday, 7:30 a.m.–7 p.m. EST; Saturday 8 a.m. –3:45 p.m. EST)
Tel.: 416-235-6556
Toll Free: 1-877-604-4567

After Hours Emergencies

For public health units, clinicians in primary care, hospitals, and long-term care facilities, and partner laboratories, contact our After Hours Duty Officer at 416-605-3113 or your local PHO laboratory.

Some laboratory locations are open for public well water specimen drop-off. Please check with your local PHO laboratory (below) to confirm if samples are accepted.

These hours are subject to change around statutory holidays. Please refer to notices on our webpages for specific holiday hours.

Laboratory Locations


Tel.: 905-385-5379

Toll Free: 1-866-282-7376

Fax: 905-385-0083

Location: 250 Fennell Avenue West Hamilton, ON L8N 3R5

Mailing Address: P.O. Box 2100, Hamilton, ON L8N 3R5


Tel.: 613-548-6630

Toll Free: 1-855-546-4745

Fax: 613-547-1185

Location: 181 Barrie Street Kingston, ON K7L 3K2

Mailing Address: P.O. Box 240, Kingston, ON K7L 4V8


Tel.: 705-325-7449

Toll Free: 1-877-611-6998

Fax: 705-329-6001

Location: 750 Memorial Avenue Orillia, ON L3V OT7

Mailing Address: P.O. Box 600, Orillia, ON L3V OT7


Tel.: 613-736-6800

Fax: 613-736-6820

Mailing Address: 2380 St. Laurent Boulevard, Ottawa, ON K1G 6C4


Tel.: 705-743-6811

Fax: 705-745-1257

Location: 99 Hospital Drive Peterborough, ON K9J 6Y8

Mailing Address: P.O. Box 265, Peterborough, ON K9J 6Y8

Sault Ste. Marie

Tel.: 705-254-7132

Toll Free: 1-800-263-0409

Fax: 705-945-6873

Mailing Address: 160 McDougald St, Sault Ste. Marie, ON P6A 3A8


Tel.: 705-564-6917

Toll Free: 1-888-564-6917

Fax: 705-564-6918

Mailing Address: 1300 Paris Street, Suite 2, Sudbury, ON P3E 6H3

Thunder Bay

Tel.: 807-622-6449

Fax: 807-622-5423

Mailing Address: 336 South Syndicate Avenue, Thunder Bay, ON P7E 1E3


Tel.: 705-267-6633

Toll Free: 1-888-267-7181

Fax: 705-360-2006

Mailing Address: 67 Wilson Avenue, Timmins, ON P4N 2S5


Tel.: 416-235-6556

Toll Free: 1-877-604-4567

Fax: 416-235-6552

Delivery Instructions: Directions to Shipping Dock for Clinical Samples

Mailing Address: 661 University Avenue, Suite 1701, Toronto, ON M5G 1M1

Updated 14 April 2022