Antimicrobial Susceptibility Testing – Anaerobes

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Antimicrobial susceptibility testing – anaerobes

Anaerobic susceptibility test are performed on isolates from sterile sites. Pure viable subculture of 24 – 48 hours growth of organism on  appropriate pre-reduced medium submitted in an anaerobic transport kit or anaerobic swab2,3

Submission and Collection Notes

1

If a swab or broth is received the turnaround time will be delayed by at least 24 hours.

2

Submit subculture of an organism on an appropriate medium that supports the growth (e.g. Blood Agar). Swab of pure culture in bacterial transport medium is an acceptable alternative.

3

Primary cultures are not acceptable; and must be processed by the originating lab. The submitter will be contacted by telephone prior to rejection of the primary sample followed by a written report to indicate that the test has been rejected.

4

Complete all fields of the Reference Bacteriology Requisition, including:

  • test(s) requested
  • culture information
  • clinical/epidemiology
  • contact information

Limitations

Mixed or non-viable cultures will not be tested. The submitter will be contacted by telephone. A written report will be issued to indicate that the test has been rejected.

Susceptibility testing on multiple isolates from the same site of infection will not be performed.

Preparation Prior to Transport

Label the specimen with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Transport a fresh subculture in anaerobic conditions to ensure viability on receipt.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Antimicrobial susceptibility testing is performed from Monday to Saturday.

Turnaround time for test results is up to 8 days from receipt by PHO laboratory.

Reporting

Results are reported to the ordering physician or health care provider as indicated on the requisition.

Test Methods

Antimicrobial Susceptibility Testing for anaerobes is performed by E-Test

contact lab

Contact Laboratory Customer Service

Laboratory Services

customerservicecentre@oahpp.ca

Updated 21 Dec 2018