Filaria – Microscopy
Submission and Collection Notes
Please note on the requisition (under other) if the patient has fever, intravascular hemolysis, hemoglobinuria, renal failure or is immunocompromised.
Indicate Country/Region and if the patient is a new immigrant or refugee or returned traveler under ‘Travel’ section of the requisition.
Submit unstained thick and thin slides AND EDTA blood. EDTA blood is required for Knott’s Concentration Procedure.
Preparation Prior to Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Place slide mailer in a biohazard bag and seal. Ship to the PHO laboratory as soon as possible.
Test Frequency and Turnaround Time (TAT)
Filaria testing is performed Monday to Friday.
Turnaround time is up to 2 days after receipt by PHO laboratory.
Results are reported to the ordering physician or health care provider as indicated on the requisition.
EDTA blood samples are examined for microfilaria using microscopy using the Knott’s Concentration Procedure.
Thick and thin smears are stained with Giemsa and examined by microscopy.