Listeria monocytogenes – Food

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Food Bacteriology1

Food – indicate if part of a meal, control, or follow up

25 grams

Sterile plastic sample bag with round wire closure

Submission and Collection Notes

1

Test selection is completed by the laboratory and is based on the information provided on the requisition.  Specific analyses may be requested using the ‘Comments’ section of the requisition.

Special Instructions

For sampling, follow the instructions found in Public Health Inspector’s Guide to Environmental Microbiology Laboratory Testing.

Shipping Instructions:

  • Ship all foods in containers with hard walls and lids secured in the closed position. 
  • Shipping containers must be labelled with the submitting organization, unique identifier and contents e.g. FOOD SAMPLES on the outside of the container. 
  • DO NOT SHIP WITH CLINICAL SPECIMENS.  Shipping containers used for food samples should be dedicated to food samples and not be used for other types of environmental samples. 
  • Ship dry foods and other shelf stable foods at ambient temperature in a closed container. 
  • Ship frozen foods in an insulated container with sufficient ice packs to maintain the frozen state. 
  • Ship perishable foods in an insulated container with sufficient cold packs to maintain a temperature as close to 4°C as possible. If ice is used, contain the ice in a manner that does not allow water contact with the samples.
  • Submit all food samples to the laboratory as soon as possible.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

The Listeria monocytogenes test is performed Monday to Saturday. 

RT-PCR turnaround time is 2 days. Culture turnaround time is up to 8 days.

 

Reporting

Results are reported to the submitting health unit as indicated on the requisition, when all analyses are completed.

Food samples that show the presence of bacteria capable of causing food-borne illness are reported to the Medical Officer of Health as per the Ontario Health Protection and Promotion Act.

Test Methods

Food and environmental swab samples are analyzed by real time polymerase chain reaction (RT-PCR, AOAC RI 011002) and/or culture for Listeria monocytogenes (Health Canada, MFHPB-30).

Interpretation

RT-PCR

  • RT-PCR will be used in the detection of Listeria monocytogenes from vegetable based products, seafood, dairy products, and environmental swab samples. Listeria monocytogenes culture will be used to confirm RT-PCR positive (Detected) and questionable test results (Refer to Culture), to determine the viability of the organism, and to perform molecular typing on isolates for epidemiological purposes.

Culture

  • The presence of Listeria monocytogenes in food is determined by culture techniques, including selective enrichment, followed by isolation on selective agar plates, and biochemical identification.

Listeria monocytogenes should be absent in ready-to-eat foods.

 

Additional Information

For outbreaks, contact your local Health Unit, your local Public Health Laboratory or the Public Health Ontario, Public Health Laboratory Customer Service at416-235-6556 or1-877-604-4567.

contact lab

Contact Laboratory Customer Service

Laboratory Services

customerservicecentre@oahpp.ca

Updated 22 Nov 2019