Q Fever – Serology

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Q-Fever serology, or Coxiella burnetti

Whole blood or serum

5.0 ml whole blood or 1.0 ml serum

Vacutainer tubes (SST)

Submission and Collection Notes

1

Indicate symptoms, exposure to farm animals, date of onset and other pertinent information such as chronic cases, granulomatous hepatitis or endocarditis on the General Test Requisition Form.

Timing of Specimen Collection

An acute (collected early after the onset of symptoms) and a convalescent (collected 2-3 weeks later) may be required for laboratory diagnosis.

Limitations

Haemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Centrifuge if using SST. Label specimens with at least two identifiers; place it in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Q Fever serology testing is performed once per week. 

Turnaround time is up to 10 days from receipt by PHO laboratory.

Reporting

Results are reported to the ordering physician or health care provider as indicated on the requisition.

Specimens that are positive for Q-Fever are reported to the Medical Officer of Health as per Health Protection and Promotion Act.

Test Methods

Q Fever (Coxiella burnetti) serology is performed using Indirect Immunofluorescence antibody (IFA) assay for IgG and IgM for both phase 1 and phase 2 antibodies.

contact lab

Contact Laboratory Customer Service

Laboratory Services

customerservicecentre@oahpp.ca

Updated 22 Oct 2019