Rubella – Immunity Serology

Testing Indications

Testing for Rubella immunity is indicated during pre-natal evaluation of pregnant women, to determine response post-vaccination, and for pre-employment screening (e.g. healthcare workers).

For symptomatic patients or post exposure, see Rubella – Diagnostic Serology or Rubella – Diagnostic RT-PCR.

If ordering as part of a Prenatal Serology work-up, see Prenatal – Serology.

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit



1.0 mL

Blood, clotted – Vacutainer tubes (SST)

Submission and Collection Notes


Two FULL red top or  serum separator tubes (SST) are sufficient for testing all Hepatitis markers, HIV, HTLV, Syphilis and Rubella.


Rubella IgG will be tested


Do NOT submit glass tubes


Haemolysed, icteric, lipemic or microbially contaminated serum is not recommended for testing.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Centrifuge if using SST. Place specimen in biohazard bag and seal. Specimens should be stored at 2-8°C following collection.

Specimens more than 10 days post collection will not be tested.

Special Instructions

Instructions for using SST tubes are found in the document titled: LAB-SD-008, Blood Collection using Serum Separator Tubes

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Rubella – Serology testing is performed daily Monday to Friday.

Turnaround time is up to 5 days from receipt by PHO laboratory.


Results are reported to the ordering physician or health care provider as indicated on the requisition.

Test Methods

Specimens for Rubella IgG (Immune status) are tested using a chemiluminescent microparticle immunoassay (CMIA) for the qualitative detection of human IgG antibody to Rubella virus.


A positive result for Rubella IgG antibodies indicates immunity to rubella virus as a result of recovery following past infection or immunization.

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Updated 13 Jan 2020