Viral Haemorrhagic Fever including Ebola Virus Disease
IMPORTANT INSTRUCTIONS Read before proceeding
If a viral haemorrhagic fever (VHF) 1 including Ebola Virus Disease (EVD) is suspected, immediately
- Inform your local/hospital infection prevention and control team, occupational health and safety team and an infectious diseases specialist.
- Inform your local/hospital laboratory management and microbiologist.
- Inform your local public health unit AND
- Following a clinical assessment of risk for EVD and/or other VHFs, consult with a PHO Laboratory Microbiologist available through the PHO Laboratory Customer Service Centre at 416-235-6556/1-877-604-4567, after-hours 416-605-3113 to request VHF including EVD testing (and concomitant malaria testing if required).
- VHF testing including EVD testing requires the concurrence of a PHO Laboratory microbiologist.
Do not collect specimens for microbiological examination before consulting with a PHO Laboratory Microbiologist.
A wide range of viruses can cause VHF. Examples of such viruses include, but are not limited to Ebola, Marburg, Lassa, Crimean-Congo hemorrhagic fever, and Rift Valley Fever virus.
For more information on Ebola and other VHFs including testing recommendations, EVD/VHF testing and shipping, visit:
Specimen Requirements
| Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
Viral Haemorrhagic Fever (VHF) testing1 including for Ebola virus disease |
See Laboratory Guidance for Viral Haemorrhagic Fevers (VHFs) |
Submission and Collection Notes
Do not collect specimens for microbiological examination before consulting with the PHO Laboratory Microbiologist. VHF testing, including for EVD, requires the concurrance of a PHO Laboratory Microbiologist. If testing is agreed to, PHO Laboratory will then provide the submitter with further direction about next steps. See Laboratory Guidance for Viral Haemorrhagic Fevers (VHFs).
Test Frequency and Turnaround Time (TAT)
Testing for EVD and other VHFs1 is performed STAT. The actual turnaround time during a specific response will be determined at the time.
As of April 29, 2019, Zaire ebolavirus PCR testing is performed at PHO Laboratory Toronto. All other VHF testing1 is performed at NML.
VHF testing is conducted using real-time PCRs targeting the individual pathogens being considered (e.g. Ebola, Marburg, Lassa). See Submission and Collection notes.
Additional Information
Footnotes:- This document does not provide information about dengue virus testing or yellow fever virus testing. See the Dengue Virus Test Information Sheet or Yellow Fever Virus Test Information Sheet.
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