Chlamydia trachomatis – Lymphogranuloma venereum (LGV) PCR

Specimen Collection and Handling

Specimen Requirements

Test Requested Required Requisition(s) Specimen Type Minimum Volume Collection Kit

Chlamydia trachomatis LGV

 

LGV / Lymphogranuloma venereum testing

Female endocervical swab

1.0 mL

CHL(C) kit/ Chlamydia trachomatis culture kit order # 390083 or 390084 or a universal transport medium (UTM)

 

Chlamydia trachomatis & Neisseria gonorrhoeae Unisex swab kit order # 300210

Chlamydia trachomatis LGV

 

LGV / Lymphogranuloma venereum testing

Male urethral swab

1.0 mL

CHL(C) kit/ Chlamydia trachomatis culture kit order # 390083 or 390084 or a universal transport medium (UTM)

 

Chlamydia trachomatis & Neisseria gonorrhoeae Unisex swab kit order # 300210

Chlamydia trachomatis LGV

 

LGV / Lymphogranuloma venereum testing

Rectal swabs

1.0 mL

CHL(C) kit/ Chlamydia trachomatis culture kit order # 390083 or 390084 or a universal transport medium (UTM)

 

Chlamydia trachomatis & Neisseria gonorrhoeae Unisex swab kit order # 300210

Chlamydia trachomatis LGV

 

LGV / Lymphogranuloma venereum testing

Urine (Aptima® specimens only)

1.0 mL

Chlamydia trachomatis & Neisseria gonorrhoeae Urine collection kit order # 300209

Submission and Collection Notes

1

Unsuitable specimens: dry swabs and swabs in bacteriological transport media (gel) are not acceptable collection kits for Chlamydia trachomatis culture and testing will not be performed.

2

Specimen collection instructions are located on the Hologic® Aptima® Unisex Swab/Urine Specimen Collection kit packaging. Refer to the instructions prior to specimen collection.

3

Do not use expired kits.

4

The patient’s date of birth is required to be provided for all NAA testing.

5

LGV must be indicated on the requisition for testing to be performed.

Preparation Prior to Transport

Label the specimen container with the patient’s full name, date of collection, and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Culture specimens should be stored at 2-8°C after collection and shipped on ice.  Transport to the laboratory as soon as possible.

Specimens should be received at the testing laboratory within 48 hours of collection. In situations of delayed transportation, specimens should be frozen at -70°C and transported on dry ice. Do not freeze sample at -20°C.

For Chlamydia culture, follow the instructions found in the Chlamydia trachomatis culture – Male or Female Kit Instruction Sheet.

After collection, Aptima® specimens must be stored and transported to the lab at 2-30°C; swabs within 60 days of collection, urines within 30 days.

Requisitions and Kit Ordering

Test Frequency and Turnaround Time (TAT)

Positive Chlamydia trachomatis specimens requesting LGV testing are shipped every Thursday to NML for testing.

Turnaround time is up to 28 days from receipt by PHO laboratory.

Reporting

Results are reported to the ordering physician or health care provider as indicated on the requisition.

Specimens that are positive for Chlamydia trachomatis are reported to the Medical Officer of Health as per the Ontario Health Protection and Promotion Act.

Preliminary positive LGV results will be reported when received from the NML.  Results are only faxed if client is registered for auto fax. A final report will be sent upon completion of the genotyping testing performed at NML.

Test Methods

The PCR test is a referred out test. Positive Chlamydia trachomatis specimens will be shipped to the National Microbiology Laboratory (NML) in Winnipeg. 

LGV testing is performed on either Chlamydia trachomatis positive cultures or from NAAT samples positive for Chlamydia trachomatis. Specimens are initially tested to screen for positivity at PHO laboratory. 

For cultures: Specimens are initially tested using standard cell culture technique; refer to Related Information. Negative Chlamydia cultures will be resulted at 48 hours and LGV testing will not be performed.

For NAAT: Specimens in Aptima® media are initially tested using nucleic acid amplification; refer to Related Information. LGV testing will not be performed on Chlamydia trachomatis negative specimens.

Refer to external resources - Chlamydia trachomatis – Lymphogranuloma venereum (LGV) PCR Molecular Detection and Genotyping. 

Data and Analysis

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Contact Laboratory Customer Service

Laboratory Services

customerservicecentre@oahpp.ca

Updated 7 Jan 2019