Hepatitis B – Serology Test Ordering Instructions

Consistent with O. Reg. 671/92 of the French Language Services Act, test ordering and instructions on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.

All sections of the General Test Requisition must be completed by authorized health care providers for each specimen submitted. Testing may be delayed or cancelled if clinical information is not provided.

Refer to Hepatitis B Virus Serology Test Information Sheet for detailed testing indications, specimens, methods and reports information.

Requisition selection(s) Tests Performed Notes Requisition Demonstration

 

Chronic Infection

 

HBsAg,

HBcAb Total (IgG+IgM)

 

 

Hep B-1 Chronic Infection

 

Acute Infection

 

HBsAg,

HBcAb Total (IgG+IgM)

 

 

HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive

 

Hep B-2 Acute Infection

 

Immune Status

 

Anti-HBs

 

 

Hep B-3 Immune Status 

 

Pre-chemotherapy screening

 

 

HBsAg,

HBcAb Total (IgG+IgM),

Anti-HBs

 

For cancer patients prior to commencing systemic therapy (chemotherapy, targeted therapy, immunotherapy)

 

Hep B-4 Pre-Chemotherapy

 

Chronic and Immunity

 

HBsAg,

HBcAb Total (IgG+IgM),

Anti-HBs

 

A reason for testing must be indicated under Testing Indication(s) / Criteria (e.g. Dialysis)

 

Hep B-5a Chronic and Immunity

 

Hep B-5b Chronic and Immunity

 

Acute and Immunity

 

HBsAg,

HBcAb Total (IgG+IgM),

Anti-HBs

 

A reason for testing must be indicated under Testing Indications(s) / Criteria (e.g. Dialysis)

 

HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive

 

Hep B-6a Acute and Immunity 

 

Hep B-6b Acute and Immunity

 

Chronic Infection

 

and

 

Test(s) Requested: HBeAg, HBeAb

 

HBsAg

HBcAb Total (IgG+IgM)

 

 

Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive

 

Hep B-7a Chronic Infection and Tests Requested

 

Hep B-7b Chronic Infection and Tests Requested

 

Acute Infection

and

Test(s) Requested: HBeAg, HBeAb

 

 

 

 

 

 

HBsAg

HBcAb Total (IgG+IgM)

 

 

Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive

 

Hep B-8a Acute Infection and Tests Requested

 

Hep B-8b Acute Infection and Tests Requested

 

Test(s) Requested: HBsAg,

Anti-HBS

 

HBsAg

Anti-HBS

 

Do not complete the Hepatitis Serology section. Enter the specific test(s) under Test(s) Requested.

Under Testing Indication(s) / Criteria,  specify “Hepatitis B Exposure” under Other

 

Hep B-9a Tests requested HBsAg_Anti-HBS

 

Hep B-9b Tests requested HBsAg_Anti-HBS

 

Test(s) Requested: HBeAg and HBeAb

or

HBcIgM

 

HBeAg/HBeAb

or

HBcIgM

 

Do not complete the Hepatitis Serology section.

Enter the specific test(s) under Test(s) Requested

 

Hep B-10 Tests requested HBsAg_HBeAb_HBcIgM

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Updated 5 Oct 2023